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A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway

The invasion-associated type III secretion system (T3SS-1) of Salmonella enterica serotype Typhimurium (S. Typhimurium) activates the transcription factor NF-κB in tissue culture cells and induces inflammatory responses in animal models through unknown mechanisms. Here we show that bacterial deliver...

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Autores principales: Marijke Keestra, A., Winter, Maria G., Klein-Douwel, Daisy, Xavier, Mariana N., Winter, Sebastian E., Kim, Anita, Tsolis, Renée M., Bäumler, Andreas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238304/
https://www.ncbi.nlm.nih.gov/pubmed/22186610
http://dx.doi.org/10.1128/mBio.00266-11
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author Marijke Keestra, A.
Winter, Maria G.
Klein-Douwel, Daisy
Xavier, Mariana N.
Winter, Sebastian E.
Kim, Anita
Tsolis, Renée M.
Bäumler, Andreas J.
author_facet Marijke Keestra, A.
Winter, Maria G.
Klein-Douwel, Daisy
Xavier, Mariana N.
Winter, Sebastian E.
Kim, Anita
Tsolis, Renée M.
Bäumler, Andreas J.
author_sort Marijke Keestra, A.
collection PubMed
description The invasion-associated type III secretion system (T3SS-1) of Salmonella enterica serotype Typhimurium (S. Typhimurium) activates the transcription factor NF-κB in tissue culture cells and induces inflammatory responses in animal models through unknown mechanisms. Here we show that bacterial delivery or ectopic expression of SipA, a T3SS-1-translocated protein, led to the activation of the NOD1/NOD2 signaling pathway and consequent RIP2-mediated induction of NF-κB-dependent inflammatory responses. SipA-mediated activation of NOD1/NOD2 signaling was independent of bacterial invasion in vitro but required an intact T3SS-1. In the mouse colitis model, SipA triggered mucosal inflammation in wild-type mice but not in NOD1/NOD2-deficient mice. These findings implicate SipA-driven activation of the NOD1/NOD2 signaling pathway as a mechanism by which the T3SS-1 induces inflammatory responses in vitro and in vivo.
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spelling pubmed-32383042011-12-20 A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway Marijke Keestra, A. Winter, Maria G. Klein-Douwel, Daisy Xavier, Mariana N. Winter, Sebastian E. Kim, Anita Tsolis, Renée M. Bäumler, Andreas J. mBio Research Article The invasion-associated type III secretion system (T3SS-1) of Salmonella enterica serotype Typhimurium (S. Typhimurium) activates the transcription factor NF-κB in tissue culture cells and induces inflammatory responses in animal models through unknown mechanisms. Here we show that bacterial delivery or ectopic expression of SipA, a T3SS-1-translocated protein, led to the activation of the NOD1/NOD2 signaling pathway and consequent RIP2-mediated induction of NF-κB-dependent inflammatory responses. SipA-mediated activation of NOD1/NOD2 signaling was independent of bacterial invasion in vitro but required an intact T3SS-1. In the mouse colitis model, SipA triggered mucosal inflammation in wild-type mice but not in NOD1/NOD2-deficient mice. These findings implicate SipA-driven activation of the NOD1/NOD2 signaling pathway as a mechanism by which the T3SS-1 induces inflammatory responses in vitro and in vivo. American Society of Microbiology 2011-12-20 /pmc/articles/PMC3238304/ /pubmed/22186610 http://dx.doi.org/10.1128/mBio.00266-11 Text en Copyright © 2011 Keestra et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Marijke Keestra, A.
Winter, Maria G.
Klein-Douwel, Daisy
Xavier, Mariana N.
Winter, Sebastian E.
Kim, Anita
Tsolis, Renée M.
Bäumler, Andreas J.
A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway
title A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway
title_full A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway
title_fullStr A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway
title_full_unstemmed A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway
title_short A Salmonella Virulence Factor Activates the NOD1/NOD2 Signaling Pathway
title_sort salmonella virulence factor activates the nod1/nod2 signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238304/
https://www.ncbi.nlm.nih.gov/pubmed/22186610
http://dx.doi.org/10.1128/mBio.00266-11
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