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Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART

Cryopreservation of human spermatozoa—introduced in the 1960's—has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner spermatozoa before assisted reproduction...

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Detalles Bibliográficos
Autores principales: Di Santo, Marlea, Tarozzi, Nicoletta, Nadalini, Marco, Borini, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238352/
https://www.ncbi.nlm.nih.gov/pubmed/22194740
http://dx.doi.org/10.1155/2012/854837
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author Di Santo, Marlea
Tarozzi, Nicoletta
Nadalini, Marco
Borini, Andrea
author_facet Di Santo, Marlea
Tarozzi, Nicoletta
Nadalini, Marco
Borini, Andrea
author_sort Di Santo, Marlea
collection PubMed
description Cryopreservation of human spermatozoa—introduced in the 1960's—has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner spermatozoa before assisted reproduction treatments and to ensure the recovery of a small number of spermatozoa in severe male factor infertility. Despite the usefulness of it, cryopreservation may lead to deleterious changes of sperm structure and function: while the effects of cryopreservation on cells are well documented, to date there is no agreement in the literature on whether or not cryopreservation affects sperm chromatin integrity or on the use of a unique and functional protocol for the freezing-thawing procedure. Therefore, sperm cryopreservation is an important component of fertility management and much of its successful application seems to affect the reproductive outcome of assisted reproduction technologies (ART): appropriate use of cryoprotectants before and sperm selection technologies after cryopreservation seem to have the greatest impact on preventing DNA fragmentation, thus improving sperm cryosurvival rates.
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spelling pubmed-32383522011-12-22 Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART Di Santo, Marlea Tarozzi, Nicoletta Nadalini, Marco Borini, Andrea Adv Urol Review Article Cryopreservation of human spermatozoa—introduced in the 1960's—has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner spermatozoa before assisted reproduction treatments and to ensure the recovery of a small number of spermatozoa in severe male factor infertility. Despite the usefulness of it, cryopreservation may lead to deleterious changes of sperm structure and function: while the effects of cryopreservation on cells are well documented, to date there is no agreement in the literature on whether or not cryopreservation affects sperm chromatin integrity or on the use of a unique and functional protocol for the freezing-thawing procedure. Therefore, sperm cryopreservation is an important component of fertility management and much of its successful application seems to affect the reproductive outcome of assisted reproduction technologies (ART): appropriate use of cryoprotectants before and sperm selection technologies after cryopreservation seem to have the greatest impact on preventing DNA fragmentation, thus improving sperm cryosurvival rates. Hindawi Publishing Corporation 2012 2011-12-13 /pmc/articles/PMC3238352/ /pubmed/22194740 http://dx.doi.org/10.1155/2012/854837 Text en Copyright © 2012 Marlea Di Santo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Di Santo, Marlea
Tarozzi, Nicoletta
Nadalini, Marco
Borini, Andrea
Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
title Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
title_full Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
title_fullStr Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
title_full_unstemmed Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
title_short Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
title_sort human sperm cryopreservation: update on techniques, effect on dna integrity, and implications for art
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238352/
https://www.ncbi.nlm.nih.gov/pubmed/22194740
http://dx.doi.org/10.1155/2012/854837
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