Cargando…

Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices

Mesenchymal stem cells are showing increasing promise in applications such as tissue engineering and cell therapy. MSC are low in number in bone marrow, and therefore in vitro expansion is often necessary. In vivo, stem cells often reside within a niche acting to protect the cells. These niches are...

Descripción completa

Detalles Bibliográficos
Autores principales: Stolzing, A., Colley, H., Scutt, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238389/
https://www.ncbi.nlm.nih.gov/pubmed/22194749
http://dx.doi.org/10.1155/2011/378034
_version_ 1782218998764535808
author Stolzing, A.
Colley, H.
Scutt, A.
author_facet Stolzing, A.
Colley, H.
Scutt, A.
author_sort Stolzing, A.
collection PubMed
description Mesenchymal stem cells are showing increasing promise in applications such as tissue engineering and cell therapy. MSC are low in number in bone marrow, and therefore in vitro expansion is often necessary. In vivo, stem cells often reside within a niche acting to protect the cells. These niches are composed of niche cells, stem cells, and extracellular matrix. When blood vessels are damaged, a fibrin clot forms as part of the wound healing response. The clot constitutes a form of stem cell niche as it appears to maintain the stem cell phenotype while supporting MSC proliferation and differentiation during healing. This is particularly appropriate as fibrin is increasingly being suggested as a scaffold meaning that fibrin-based tissue engineering may to some extent recapitulate wound healing. Here, we describe how fibrin modulates the clonogenic capacity of MSC derived from young/old human donors and normal/diabetic rats. Fibrin was prepared using different concentrations to modulate the stiffness of the substrate. MSC were expanded on these scaffolds and analysed. MSC showed an increased self-renewal on soft surfaces. Old and diabetic cells lost the ability to react to these signals and can no longer adapt to the changed environment.
format Online
Article
Text
id pubmed-3238389
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-32383892011-12-22 Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices Stolzing, A. Colley, H. Scutt, A. Int J Biomater Research Article Mesenchymal stem cells are showing increasing promise in applications such as tissue engineering and cell therapy. MSC are low in number in bone marrow, and therefore in vitro expansion is often necessary. In vivo, stem cells often reside within a niche acting to protect the cells. These niches are composed of niche cells, stem cells, and extracellular matrix. When blood vessels are damaged, a fibrin clot forms as part of the wound healing response. The clot constitutes a form of stem cell niche as it appears to maintain the stem cell phenotype while supporting MSC proliferation and differentiation during healing. This is particularly appropriate as fibrin is increasingly being suggested as a scaffold meaning that fibrin-based tissue engineering may to some extent recapitulate wound healing. Here, we describe how fibrin modulates the clonogenic capacity of MSC derived from young/old human donors and normal/diabetic rats. Fibrin was prepared using different concentrations to modulate the stiffness of the substrate. MSC were expanded on these scaffolds and analysed. MSC showed an increased self-renewal on soft surfaces. Old and diabetic cells lost the ability to react to these signals and can no longer adapt to the changed environment. Hindawi Publishing Corporation 2011 2011-12-13 /pmc/articles/PMC3238389/ /pubmed/22194749 http://dx.doi.org/10.1155/2011/378034 Text en Copyright © 2011 A. Stolzing et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Stolzing, A.
Colley, H.
Scutt, A.
Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices
title Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices
title_full Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices
title_fullStr Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices
title_full_unstemmed Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices
title_short Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices
title_sort effect of age and diabetes on the response of mesenchymal progenitor cells to fibrin matrices
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238389/
https://www.ncbi.nlm.nih.gov/pubmed/22194749
http://dx.doi.org/10.1155/2011/378034
work_keys_str_mv AT stolzinga effectofageanddiabetesontheresponseofmesenchymalprogenitorcellstofibrinmatrices
AT colleyh effectofageanddiabetesontheresponseofmesenchymalprogenitorcellstofibrinmatrices
AT scutta effectofageanddiabetesontheresponseofmesenchymalprogenitorcellstofibrinmatrices