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Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD

A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on...

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Autores principales: Huang, Da-Ming, Zhang, Tian-Zhen, Cui, Feng-Jie, Sun, Wen-Jing, Zhao, Li-Ming, Yang, Meng-Yi, Wang, Ya-Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238810/
https://www.ncbi.nlm.nih.gov/pubmed/22203787
http://dx.doi.org/10.1155/2011/917232
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author Huang, Da-Ming
Zhang, Tian-Zhen
Cui, Feng-Jie
Sun, Wen-Jing
Zhao, Li-Ming
Yang, Meng-Yi
Wang, Ya-Juan
author_facet Huang, Da-Ming
Zhang, Tian-Zhen
Cui, Feng-Jie
Sun, Wen-Jing
Zhao, Li-Ming
Yang, Meng-Yi
Wang, Ya-Juan
author_sort Huang, Da-Ming
collection PubMed
description A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) were set as follows: detection wavelength of 280 nm, mobile phase of water and methanol gradient elution, temperature for the chromatographic column of 30°C, flow rate of mobile phase of 0.8 mL/min, sample injection volume of 5 μL, and elution time of 40 min. The MS conditions were set as follows: the flow rate of sheath gas, aux gas, and sweep gas were kept at 35 arb, 5 arb, and 0 arb, respectively. The temperature of capillary was held at 300°C, and capillary voltage was set at 30.00 V. Tube lens were performed at 100.00 V. The proposed method was validated by linearity (r(2) ≥ 0.9910), average recovery (94.93%, RSD1.21%), precision (RSD ≤ 1.31%), limit of detection, and limit of quantification (LOD 0.1~0.12 mg/L, LOQ 0.5~0.67 mg/L), which proved to be affordable for simultaneously determining canrenone and its bio-transformed product 11-α-hydroxy-canrenone.
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spelling pubmed-32388102011-12-27 Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD Huang, Da-Ming Zhang, Tian-Zhen Cui, Feng-Jie Sun, Wen-Jing Zhao, Li-Ming Yang, Meng-Yi Wang, Ya-Juan J Biomed Biotechnol Research Article A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) were set as follows: detection wavelength of 280 nm, mobile phase of water and methanol gradient elution, temperature for the chromatographic column of 30°C, flow rate of mobile phase of 0.8 mL/min, sample injection volume of 5 μL, and elution time of 40 min. The MS conditions were set as follows: the flow rate of sheath gas, aux gas, and sweep gas were kept at 35 arb, 5 arb, and 0 arb, respectively. The temperature of capillary was held at 300°C, and capillary voltage was set at 30.00 V. Tube lens were performed at 100.00 V. The proposed method was validated by linearity (r(2) ≥ 0.9910), average recovery (94.93%, RSD1.21%), precision (RSD ≤ 1.31%), limit of detection, and limit of quantification (LOD 0.1~0.12 mg/L, LOQ 0.5~0.67 mg/L), which proved to be affordable for simultaneously determining canrenone and its bio-transformed product 11-α-hydroxy-canrenone. Hindawi Publishing Corporation 2011 2011-12-13 /pmc/articles/PMC3238810/ /pubmed/22203787 http://dx.doi.org/10.1155/2011/917232 Text en Copyright © 2011 Da-Ming Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Da-Ming
Zhang, Tian-Zhen
Cui, Feng-Jie
Sun, Wen-Jing
Zhao, Li-Ming
Yang, Meng-Yi
Wang, Ya-Juan
Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
title Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
title_full Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
title_fullStr Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
title_full_unstemmed Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
title_short Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
title_sort simultaneous identification and quantification of canrenone and 11-α-hydroxy-canrenone by lc-ms and hplc-uvd
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238810/
https://www.ncbi.nlm.nih.gov/pubmed/22203787
http://dx.doi.org/10.1155/2011/917232
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