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Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning

BACKGROUND: The advent of genomics-based technologies has revolutionized many fields of biological enquiry. However, chromosome walking or flanking sequence cloning is still a necessary and important procedure to determining gene structure. Such methods are used to identify T-DNA insertion sites and...

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Detalles Bibliográficos
Autores principales:	Wang, Zhen, Ye, Shafei, Li, Jingjing, Zheng, Bo, Bao, Manzhu, Ning, Guogui
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3239319/ https://www.ncbi.nlm.nih.gov/pubmed/22093809 http://dx.doi.org/10.1186/1472-6750-11-109

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