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Extracting Fluorescent Reporter Time Courses of Cell Lineages from High-Throughput Microscopy at Low Temporal Resolution
The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the expression of fluorescent reporters through cell d...
Autores principales: | Downey, Mike J., Jeziorska, Danuta M., Ott, Sascha, Tamai, T. Katherine, Koentges, Georgy, Vance, Keith W., Bretschneider, Till |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240619/ https://www.ncbi.nlm.nih.gov/pubmed/22194797 http://dx.doi.org/10.1371/journal.pone.0027886 |
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