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The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF

During development, axons of neurons in the mammalian central nervous system lose their ability to regenerate. To study the regeneration process, axons of mouse hippocampal neurons were partially damaged by an UVA laser dissector system. The possibility to deliver very low average power to the sampl...

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Autores principales: Difato, Francesco, Tsushima, Hanako, Pesce, Mattia, Benfenati, Fabio, Blau, Axel, Chieregatti, Evelina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240951/
https://www.ncbi.nlm.nih.gov/pubmed/22355698
http://dx.doi.org/10.1038/srep00183
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author Difato, Francesco
Tsushima, Hanako
Pesce, Mattia
Benfenati, Fabio
Blau, Axel
Chieregatti, Evelina
author_facet Difato, Francesco
Tsushima, Hanako
Pesce, Mattia
Benfenati, Fabio
Blau, Axel
Chieregatti, Evelina
author_sort Difato, Francesco
collection PubMed
description During development, axons of neurons in the mammalian central nervous system lose their ability to regenerate. To study the regeneration process, axons of mouse hippocampal neurons were partially damaged by an UVA laser dissector system. The possibility to deliver very low average power to the sample reduced the collateral thermal damage and allowed studying axonal regeneration of mouse neurons during early days in vitro. Force spectroscopy measurements were performed during and after axon ablation with a bead attached to the axonal membrane and held in an optical trap. With this approach, we quantified the adhesion of the axon to the substrate and the viscoelastic properties of the membrane during regeneration. The reorganization and regeneration of the axon was documented by long-term live imaging. Here we demonstrate that BDNF regulates neuronal adhesion and favors the formation of actin waves during regeneration after axonal lesion.
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spelling pubmed-32409512011-12-22 The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF Difato, Francesco Tsushima, Hanako Pesce, Mattia Benfenati, Fabio Blau, Axel Chieregatti, Evelina Sci Rep Article During development, axons of neurons in the mammalian central nervous system lose their ability to regenerate. To study the regeneration process, axons of mouse hippocampal neurons were partially damaged by an UVA laser dissector system. The possibility to deliver very low average power to the sample reduced the collateral thermal damage and allowed studying axonal regeneration of mouse neurons during early days in vitro. Force spectroscopy measurements were performed during and after axon ablation with a bead attached to the axonal membrane and held in an optical trap. With this approach, we quantified the adhesion of the axon to the substrate and the viscoelastic properties of the membrane during regeneration. The reorganization and regeneration of the axon was documented by long-term live imaging. Here we demonstrate that BDNF regulates neuronal adhesion and favors the formation of actin waves during regeneration after axonal lesion. Nature Publishing Group 2011-12-06 /pmc/articles/PMC3240951/ /pubmed/22355698 http://dx.doi.org/10.1038/srep00183 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Difato, Francesco
Tsushima, Hanako
Pesce, Mattia
Benfenati, Fabio
Blau, Axel
Chieregatti, Evelina
The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF
title The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF
title_full The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF
title_fullStr The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF
title_full_unstemmed The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF
title_short The formation of actin waves during regeneration after axonal lesion is enhanced by BDNF
title_sort formation of actin waves during regeneration after axonal lesion is enhanced by bdnf
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240951/
https://www.ncbi.nlm.nih.gov/pubmed/22355698
http://dx.doi.org/10.1038/srep00183
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