Generation of mouse ES cell lines engineered for the forced induction of transcription factors

Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7–10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction...

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Autores principales: Correa-Cerro, Lina S., Piao, Yulan, Sharov, Alexei A., Nishiyama, Akira, Cadet, Jean S., Yu, Hong, Sharova, Lioudmila V., Xin, Li, Hoang, Hien G., Thomas, Marshall, Qian, Yong, Dudekula, Dawood B., Meyers, Emily, Binder, Bernard Y., Mowrer, Gregory, Bassey, Uwem, Longo, Dan L., Schlessinger, David, Ko, Minoru S. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240988/
https://www.ncbi.nlm.nih.gov/pubmed/22355682
http://dx.doi.org/10.1038/srep00167
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author Correa-Cerro, Lina S.
Piao, Yulan
Sharov, Alexei A.
Nishiyama, Akira
Cadet, Jean S.
Yu, Hong
Sharova, Lioudmila V.
Xin, Li
Hoang, Hien G.
Thomas, Marshall
Qian, Yong
Dudekula, Dawood B.
Meyers, Emily
Binder, Bernard Y.
Mowrer, Gregory
Bassey, Uwem
Longo, Dan L.
Schlessinger, David
Ko, Minoru S. H.
author_facet Correa-Cerro, Lina S.
Piao, Yulan
Sharov, Alexei A.
Nishiyama, Akira
Cadet, Jean S.
Yu, Hong
Sharova, Lioudmila V.
Xin, Li
Hoang, Hien G.
Thomas, Marshall
Qian, Yong
Dudekula, Dawood B.
Meyers, Emily
Binder, Bernard Y.
Mowrer, Gregory
Bassey, Uwem
Longo, Dan L.
Schlessinger, David
Ko, Minoru S. H.
author_sort Correa-Cerro, Lina S.
collection PubMed
description Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7–10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.
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spelling pubmed-32409882011-12-22 Generation of mouse ES cell lines engineered for the forced induction of transcription factors Correa-Cerro, Lina S. Piao, Yulan Sharov, Alexei A. Nishiyama, Akira Cadet, Jean S. Yu, Hong Sharova, Lioudmila V. Xin, Li Hoang, Hien G. Thomas, Marshall Qian, Yong Dudekula, Dawood B. Meyers, Emily Binder, Bernard Y. Mowrer, Gregory Bassey, Uwem Longo, Dan L. Schlessinger, David Ko, Minoru S. H. Sci Rep Article Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7–10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource. Nature Publishing Group 2011-11-23 /pmc/articles/PMC3240988/ /pubmed/22355682 http://dx.doi.org/10.1038/srep00167 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Correa-Cerro, Lina S.
Piao, Yulan
Sharov, Alexei A.
Nishiyama, Akira
Cadet, Jean S.
Yu, Hong
Sharova, Lioudmila V.
Xin, Li
Hoang, Hien G.
Thomas, Marshall
Qian, Yong
Dudekula, Dawood B.
Meyers, Emily
Binder, Bernard Y.
Mowrer, Gregory
Bassey, Uwem
Longo, Dan L.
Schlessinger, David
Ko, Minoru S. H.
Generation of mouse ES cell lines engineered for the forced induction of transcription factors
title Generation of mouse ES cell lines engineered for the forced induction of transcription factors
title_full Generation of mouse ES cell lines engineered for the forced induction of transcription factors
title_fullStr Generation of mouse ES cell lines engineered for the forced induction of transcription factors
title_full_unstemmed Generation of mouse ES cell lines engineered for the forced induction of transcription factors
title_short Generation of mouse ES cell lines engineered for the forced induction of transcription factors
title_sort generation of mouse es cell lines engineered for the forced induction of transcription factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240988/
https://www.ncbi.nlm.nih.gov/pubmed/22355682
http://dx.doi.org/10.1038/srep00167
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