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Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing
The N-terminal tail of histone H2B is believed to be involved in gene silencing, but how it exerts its function remains elusive. Here, we report the biochemical characterization of p14ARF tumor suppressor as a transcriptional repressor that selectively recognizes the unacetylated H2B tails on nucleo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241654/ https://www.ncbi.nlm.nih.gov/pubmed/21846774 http://dx.doi.org/10.1093/nar/gkr642 |
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author | Choi, Jongkyu Kim, Hyunjung Kim, Kyunghwan Lee, Bomi Lu, Wange An, Woojin |
author_facet | Choi, Jongkyu Kim, Hyunjung Kim, Kyunghwan Lee, Bomi Lu, Wange An, Woojin |
author_sort | Choi, Jongkyu |
collection | PubMed |
description | The N-terminal tail of histone H2B is believed to be involved in gene silencing, but how it exerts its function remains elusive. Here, we report the biochemical characterization of p14ARF tumor suppressor as a transcriptional repressor that selectively recognizes the unacetylated H2B tails on nucleosomes. The p14ARF–H2B tail interaction is functional, as the antagonistic effect of p14ARF on chromatin transcription is lost upon deletion or acetylation of H2B tails. Gene expression profiling and chromatin immunoprecipitation studies emphasize the significance of H2B deacetylation and p14ARF recruitment in establishing a repressive environment over the cell cycle regulatory genes. Moreover, HDAC1-mediated H2B deacetylation, especially at K20, constitutes an essential step in tethering p14ARF near target promoters. Our results thus reveal a hitherto unknown role of p14ARF in the regulation of chromatin transcription, as well as molecular mechanisms governing the repressive action of p14ARF. |
format | Online Article Text |
id | pubmed-3241654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32416542011-12-19 Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing Choi, Jongkyu Kim, Hyunjung Kim, Kyunghwan Lee, Bomi Lu, Wange An, Woojin Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics The N-terminal tail of histone H2B is believed to be involved in gene silencing, but how it exerts its function remains elusive. Here, we report the biochemical characterization of p14ARF tumor suppressor as a transcriptional repressor that selectively recognizes the unacetylated H2B tails on nucleosomes. The p14ARF–H2B tail interaction is functional, as the antagonistic effect of p14ARF on chromatin transcription is lost upon deletion or acetylation of H2B tails. Gene expression profiling and chromatin immunoprecipitation studies emphasize the significance of H2B deacetylation and p14ARF recruitment in establishing a repressive environment over the cell cycle regulatory genes. Moreover, HDAC1-mediated H2B deacetylation, especially at K20, constitutes an essential step in tethering p14ARF near target promoters. Our results thus reveal a hitherto unknown role of p14ARF in the regulation of chromatin transcription, as well as molecular mechanisms governing the repressive action of p14ARF. Oxford University Press 2011-11 2011-08-16 /pmc/articles/PMC3241654/ /pubmed/21846774 http://dx.doi.org/10.1093/nar/gkr642 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene Regulation, Chromatin and Epigenetics Choi, Jongkyu Kim, Hyunjung Kim, Kyunghwan Lee, Bomi Lu, Wange An, Woojin Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing |
title | Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing |
title_full | Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing |
title_fullStr | Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing |
title_full_unstemmed | Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing |
title_short | Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing |
title_sort | selective requirement of h2b n-terminal tail for p14arf-induced chromatin silencing |
topic | Gene Regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241654/ https://www.ncbi.nlm.nih.gov/pubmed/21846774 http://dx.doi.org/10.1093/nar/gkr642 |
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