Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil

A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus...

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Detalles Bibliográficos
Autores principales: Showmaker, Kurt, Lawrence, Gary W., Lu, Shien, Balbalian, Clarissa, Klink, Vincent P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241691/
https://www.ncbi.nlm.nih.gov/pubmed/22194958
http://dx.doi.org/10.1371/journal.pone.0028954
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author Showmaker, Kurt
Lawrence, Gary W.
Lu, Shien
Balbalian, Clarissa
Klink, Vincent P.
author_facet Showmaker, Kurt
Lawrence, Gary W.
Lu, Shien
Balbalian, Clarissa
Klink, Vincent P.
author_sort Showmaker, Kurt
collection PubMed
description A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.
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spelling pubmed-32416912011-12-22 Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil Showmaker, Kurt Lawrence, Gary W. Lu, Shien Balbalian, Clarissa Klink, Vincent P. PLoS One Research Article A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil. Public Library of Science 2011-12-16 /pmc/articles/PMC3241691/ /pubmed/22194958 http://dx.doi.org/10.1371/journal.pone.0028954 Text en Showmaker et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Showmaker, Kurt
Lawrence, Gary W.
Lu, Shien
Balbalian, Clarissa
Klink, Vincent P.
Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil
title Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil
title_full Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil
title_fullStr Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil
title_full_unstemmed Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil
title_short Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil
title_sort quantitative field testing rotylenchulus reniformis dna from metagenomic samples isolated directly from soil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241691/
https://www.ncbi.nlm.nih.gov/pubmed/22194958
http://dx.doi.org/10.1371/journal.pone.0028954
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