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Adaption of Seasonal H1N1 Influenza Virus in Mice
The experimental infection of a mouse lung with influenza A virus has proven to be an invaluable model for studying the mechanisms of viral adaptation and virulence. The mouse adaption of human influenza A virus can result in mutations in the HA and other proteins, which is associated with increased...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241702/ https://www.ncbi.nlm.nih.gov/pubmed/22194944 http://dx.doi.org/10.1371/journal.pone.0028901 |
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author | Xu, Lili Bao, Linlin Li, Fengdi Lv, Qi Ma, Yila Zhou, Jiangfang Xu, Yanfeng Deng, Wei Zhan, Lingjun Zhu, Hua Ma, Chunmei Shu, Yuelong Qin, Chuan |
author_facet | Xu, Lili Bao, Linlin Li, Fengdi Lv, Qi Ma, Yila Zhou, Jiangfang Xu, Yanfeng Deng, Wei Zhan, Lingjun Zhu, Hua Ma, Chunmei Shu, Yuelong Qin, Chuan |
author_sort | Xu, Lili |
collection | PubMed |
description | The experimental infection of a mouse lung with influenza A virus has proven to be an invaluable model for studying the mechanisms of viral adaptation and virulence. The mouse adaption of human influenza A virus can result in mutations in the HA and other proteins, which is associated with increased virulence in mouse lungs. In this study, a mouse-adapted seasonal H1N1 virus was obtained through serial lung-to-lung passages and had significantly increased virulence and pathogenicity in mice. Genetic analysis indicated that the increased virulence of the mouse-adapted virus was attributed to incremental acquisition of three mutations in the HA protein (T89I, N125T, and D221G). However, the mouse adaption of influenza A virus did not change the specificity and affinity of receptor binding and the pH-dependent membrane fusion of HA, as well as the in vitro replication in MDCK cells. Notably, infection with the mouse adapted virus induced severe lymphopenia and modulated cytokine and chemokine responses in mice. Apparently, mouse adaption of human influenza A virus may change the ability to replicate in mouse lungs, which induces strong immune responses and inflammation in mice. Therefore, our findings may provide new insights into understanding the mechanisms underlying the mouse adaption and pathogenicity of highly virulent influenza viruses. |
format | Online Article Text |
id | pubmed-3241702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32417022011-12-22 Adaption of Seasonal H1N1 Influenza Virus in Mice Xu, Lili Bao, Linlin Li, Fengdi Lv, Qi Ma, Yila Zhou, Jiangfang Xu, Yanfeng Deng, Wei Zhan, Lingjun Zhu, Hua Ma, Chunmei Shu, Yuelong Qin, Chuan PLoS One Research Article The experimental infection of a mouse lung with influenza A virus has proven to be an invaluable model for studying the mechanisms of viral adaptation and virulence. The mouse adaption of human influenza A virus can result in mutations in the HA and other proteins, which is associated with increased virulence in mouse lungs. In this study, a mouse-adapted seasonal H1N1 virus was obtained through serial lung-to-lung passages and had significantly increased virulence and pathogenicity in mice. Genetic analysis indicated that the increased virulence of the mouse-adapted virus was attributed to incremental acquisition of three mutations in the HA protein (T89I, N125T, and D221G). However, the mouse adaption of influenza A virus did not change the specificity and affinity of receptor binding and the pH-dependent membrane fusion of HA, as well as the in vitro replication in MDCK cells. Notably, infection with the mouse adapted virus induced severe lymphopenia and modulated cytokine and chemokine responses in mice. Apparently, mouse adaption of human influenza A virus may change the ability to replicate in mouse lungs, which induces strong immune responses and inflammation in mice. Therefore, our findings may provide new insights into understanding the mechanisms underlying the mouse adaption and pathogenicity of highly virulent influenza viruses. Public Library of Science 2011-12-16 /pmc/articles/PMC3241702/ /pubmed/22194944 http://dx.doi.org/10.1371/journal.pone.0028901 Text en Xu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xu, Lili Bao, Linlin Li, Fengdi Lv, Qi Ma, Yila Zhou, Jiangfang Xu, Yanfeng Deng, Wei Zhan, Lingjun Zhu, Hua Ma, Chunmei Shu, Yuelong Qin, Chuan Adaption of Seasonal H1N1 Influenza Virus in Mice |
title | Adaption of Seasonal H1N1 Influenza Virus in Mice |
title_full | Adaption of Seasonal H1N1 Influenza Virus in Mice |
title_fullStr | Adaption of Seasonal H1N1 Influenza Virus in Mice |
title_full_unstemmed | Adaption of Seasonal H1N1 Influenza Virus in Mice |
title_short | Adaption of Seasonal H1N1 Influenza Virus in Mice |
title_sort | adaption of seasonal h1n1 influenza virus in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241702/ https://www.ncbi.nlm.nih.gov/pubmed/22194944 http://dx.doi.org/10.1371/journal.pone.0028901 |
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