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Development of immunoassays for the detection of kanamycin in veterinary fields
Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reacti...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242100/ https://www.ncbi.nlm.nih.gov/pubmed/16645333 http://dx.doi.org/10.4142/jvs.2006.7.2.111 |
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author | Jin, Yong Jang, Jin-Wook Han, Chang-Hoon Lee, Mun-Han |
author_facet | Jin, Yong Jang, Jin-Wook Han, Chang-Hoon Lee, Mun-Han |
author_sort | Jin, Yong |
collection | PubMed |
description | Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of kanamycin were determined to be 1.1 ng/ml in PBS, 1.4 ng/ml in plasma, and 1.0 ng/ml in milk. The concentration of intramuscularly injected kanamycin was successfully monitored in rabbit plasma with competitive direct ELISA. Based on the colloidal gold-based immunochromatographic assay, the detection limits of kanamycin were estimated to be about 6-8 ng/ml in PBS, plasma, and milk. The immunochromatographic assay would be suitable for rapid and simple screening of kanamycin residues in veterinary medicine. Screened positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA. Therefore, the assays developed in this study could be used to complement each other as well as other laboratory findings. Moreover, instead of slaughtering the animals to obtain test samples, these methods could be applied to determine kanamycin concentration in the plasma of live animals. |
format | Online Article Text |
id | pubmed-3242100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32421002011-12-22 Development of immunoassays for the detection of kanamycin in veterinary fields Jin, Yong Jang, Jin-Wook Han, Chang-Hoon Lee, Mun-Han J Vet Sci Original Article Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of kanamycin were determined to be 1.1 ng/ml in PBS, 1.4 ng/ml in plasma, and 1.0 ng/ml in milk. The concentration of intramuscularly injected kanamycin was successfully monitored in rabbit plasma with competitive direct ELISA. Based on the colloidal gold-based immunochromatographic assay, the detection limits of kanamycin were estimated to be about 6-8 ng/ml in PBS, plasma, and milk. The immunochromatographic assay would be suitable for rapid and simple screening of kanamycin residues in veterinary medicine. Screened positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA. Therefore, the assays developed in this study could be used to complement each other as well as other laboratory findings. Moreover, instead of slaughtering the animals to obtain test samples, these methods could be applied to determine kanamycin concentration in the plasma of live animals. The Korean Society of Veterinary Science 2006-06 2006-06-30 /pmc/articles/PMC3242100/ /pubmed/16645333 http://dx.doi.org/10.4142/jvs.2006.7.2.111 Text en Copyright © 2006 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jin, Yong Jang, Jin-Wook Han, Chang-Hoon Lee, Mun-Han Development of immunoassays for the detection of kanamycin in veterinary fields |
title | Development of immunoassays for the detection of kanamycin in veterinary fields |
title_full | Development of immunoassays for the detection of kanamycin in veterinary fields |
title_fullStr | Development of immunoassays for the detection of kanamycin in veterinary fields |
title_full_unstemmed | Development of immunoassays for the detection of kanamycin in veterinary fields |
title_short | Development of immunoassays for the detection of kanamycin in veterinary fields |
title_sort | development of immunoassays for the detection of kanamycin in veterinary fields |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242100/ https://www.ncbi.nlm.nih.gov/pubmed/16645333 http://dx.doi.org/10.4142/jvs.2006.7.2.111 |
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