Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India

The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were...

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Detalles Bibliográficos
Autores principales: Singh, J. P. N., Verma, Rishendra, Chaudhuri, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2006
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242112/
https://www.ncbi.nlm.nih.gov/pubmed/16645345
http://dx.doi.org/10.4142/jvs.2006.7.2.181
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author Singh, J. P. N.
Verma, Rishendra
Chaudhuri, P.
author_facet Singh, J. P. N.
Verma, Rishendra
Chaudhuri, P.
author_sort Singh, J. P. N.
collection PubMed
description The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates, and it was found to be a valuable alternative epidemiological tool. In addition, the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied.
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spelling pubmed-32421122011-12-22 Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India Singh, J. P. N. Verma, Rishendra Chaudhuri, P. J Vet Sci Original Article The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates, and it was found to be a valuable alternative epidemiological tool. In addition, the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied. The Korean Society of Veterinary Science 2006-06 2006-06-30 /pmc/articles/PMC3242112/ /pubmed/16645345 http://dx.doi.org/10.4142/jvs.2006.7.2.181 Text en Copyright © 2006 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Singh, J. P. N.
Verma, Rishendra
Chaudhuri, P.
Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
title Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
title_full Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
title_fullStr Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
title_full_unstemmed Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
title_short Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
title_sort random amplified polymorphic dna (rapd) analysis of mycobacterium tuberculosis strains in india
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242112/
https://www.ncbi.nlm.nih.gov/pubmed/16645345
http://dx.doi.org/10.4142/jvs.2006.7.2.181
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