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The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates

Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accura...

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Autores principales: Kim, Tae Jung, Lee, Jae Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242128/
https://www.ncbi.nlm.nih.gov/pubmed/16871023
http://dx.doi.org/10.4142/jvs.2006.7.3.277
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author Kim, Tae Jung
Lee, Jae Il
author_facet Kim, Tae Jung
Lee, Jae Il
author_sort Kim, Tae Jung
collection PubMed
description Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine.
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spelling pubmed-32421282011-12-22 The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates Kim, Tae Jung Lee, Jae Il J Vet Sci Original Article Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine. The Korean Society of Veterinary Science 2006-09 2006-09-30 /pmc/articles/PMC3242128/ /pubmed/16871023 http://dx.doi.org/10.4142/jvs.2006.7.3.277 Text en Copyright © 2006 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Tae Jung
Lee, Jae Il
The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
title The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
title_full The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
title_fullStr The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
title_full_unstemmed The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
title_short The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
title_sort 23s rrna gene pcr-rflp used for characterization of porcine intestinal spirochete isolates
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242128/
https://www.ncbi.nlm.nih.gov/pubmed/16871023
http://dx.doi.org/10.4142/jvs.2006.7.3.277
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