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Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone

We report on the comparative genomics and characterization of the virulence phenotypes of four S. pneumoniae strains that belong to the multidrug resistant clone PMEN1 (Spain(23F) ST81). Strains SV35-T23 and SV36-T3 were recovered in 1996 from the nasopharynx of patients at an AIDS hospice in New Yo...

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Autores principales: Hiller, N. Luisa, Eutsey, Rory A., Powell, Evan, Earl, Joshua P., Janto, Benjamin, Martin, Darren P., Dawid, Suzanne, Ahmed, Azad, Longwell, Mark J., Dahlgren, Margaret E., Ezzo, Suzanne, Tettelin, Herve, Daugherty, Sean C., Mitchell, Timothy J., Hillman, Todd A., Buchinsky, Farrel J., Tomasz, Alexander, de Lencastre, Herminia, Sá-Leão, Raquel, Post, J. Christopher, Hu, Fen Z., Ehrlich, Garth D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242761/
https://www.ncbi.nlm.nih.gov/pubmed/22205975
http://dx.doi.org/10.1371/journal.pone.0028850
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author Hiller, N. Luisa
Eutsey, Rory A.
Powell, Evan
Earl, Joshua P.
Janto, Benjamin
Martin, Darren P.
Dawid, Suzanne
Ahmed, Azad
Longwell, Mark J.
Dahlgren, Margaret E.
Ezzo, Suzanne
Tettelin, Herve
Daugherty, Sean C.
Mitchell, Timothy J.
Hillman, Todd A.
Buchinsky, Farrel J.
Tomasz, Alexander
de Lencastre, Herminia
Sá-Leão, Raquel
Post, J. Christopher
Hu, Fen Z.
Ehrlich, Garth D.
author_facet Hiller, N. Luisa
Eutsey, Rory A.
Powell, Evan
Earl, Joshua P.
Janto, Benjamin
Martin, Darren P.
Dawid, Suzanne
Ahmed, Azad
Longwell, Mark J.
Dahlgren, Margaret E.
Ezzo, Suzanne
Tettelin, Herve
Daugherty, Sean C.
Mitchell, Timothy J.
Hillman, Todd A.
Buchinsky, Farrel J.
Tomasz, Alexander
de Lencastre, Herminia
Sá-Leão, Raquel
Post, J. Christopher
Hu, Fen Z.
Ehrlich, Garth D.
author_sort Hiller, N. Luisa
collection PubMed
description We report on the comparative genomics and characterization of the virulence phenotypes of four S. pneumoniae strains that belong to the multidrug resistant clone PMEN1 (Spain(23F) ST81). Strains SV35-T23 and SV36-T3 were recovered in 1996 from the nasopharynx of patients at an AIDS hospice in New York. Strain SV36-T3 expressed capsule type 3 which is unusual for this clone and represents the product of an in vivo capsular switch event. A third PMEN1 isolate – PN4595-T23 – was recovered in 1996 from the nasopharynx of a child attending day care in Portugal, and a fourth strain – ATCC700669 – was originally isolated from a patient with pneumococcal disease in Spain in 1984. We compared the genomes among four PMEN1 strains and 47 previously sequenced pneumococcal isolates for gene possession differences and allelic variations within core genes. In contrast to the 47 strains – representing a variety of clonal types – the four PMEN1 strains grouped closely together, demonstrating high genomic conservation within this lineage relative to the rest of the species. In the four PMEN1 strains allelic and gene possession differences were clustered into 18 genomic regions including the capsule, the blp bacteriocins, erythromycin resistance, the MM1-2008 prophage and multiple cell wall anchored proteins. In spite of their genomic similarity, the high resolution chinchilla model was able to detect variations in virulence properties of the PMEN1 strains highlighting how small genic or allelic variation can lead to significant changes in pathogenicity and making this set of strains ideal for the identification of novel virulence determinants.
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spelling pubmed-32427612011-12-28 Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone Hiller, N. Luisa Eutsey, Rory A. Powell, Evan Earl, Joshua P. Janto, Benjamin Martin, Darren P. Dawid, Suzanne Ahmed, Azad Longwell, Mark J. Dahlgren, Margaret E. Ezzo, Suzanne Tettelin, Herve Daugherty, Sean C. Mitchell, Timothy J. Hillman, Todd A. Buchinsky, Farrel J. Tomasz, Alexander de Lencastre, Herminia Sá-Leão, Raquel Post, J. Christopher Hu, Fen Z. Ehrlich, Garth D. PLoS One Research Article We report on the comparative genomics and characterization of the virulence phenotypes of four S. pneumoniae strains that belong to the multidrug resistant clone PMEN1 (Spain(23F) ST81). Strains SV35-T23 and SV36-T3 were recovered in 1996 from the nasopharynx of patients at an AIDS hospice in New York. Strain SV36-T3 expressed capsule type 3 which is unusual for this clone and represents the product of an in vivo capsular switch event. A third PMEN1 isolate – PN4595-T23 – was recovered in 1996 from the nasopharynx of a child attending day care in Portugal, and a fourth strain – ATCC700669 – was originally isolated from a patient with pneumococcal disease in Spain in 1984. We compared the genomes among four PMEN1 strains and 47 previously sequenced pneumococcal isolates for gene possession differences and allelic variations within core genes. In contrast to the 47 strains – representing a variety of clonal types – the four PMEN1 strains grouped closely together, demonstrating high genomic conservation within this lineage relative to the rest of the species. In the four PMEN1 strains allelic and gene possession differences were clustered into 18 genomic regions including the capsule, the blp bacteriocins, erythromycin resistance, the MM1-2008 prophage and multiple cell wall anchored proteins. In spite of their genomic similarity, the high resolution chinchilla model was able to detect variations in virulence properties of the PMEN1 strains highlighting how small genic or allelic variation can lead to significant changes in pathogenicity and making this set of strains ideal for the identification of novel virulence determinants. Public Library of Science 2011-12-19 /pmc/articles/PMC3242761/ /pubmed/22205975 http://dx.doi.org/10.1371/journal.pone.0028850 Text en Hiller et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hiller, N. Luisa
Eutsey, Rory A.
Powell, Evan
Earl, Joshua P.
Janto, Benjamin
Martin, Darren P.
Dawid, Suzanne
Ahmed, Azad
Longwell, Mark J.
Dahlgren, Margaret E.
Ezzo, Suzanne
Tettelin, Herve
Daugherty, Sean C.
Mitchell, Timothy J.
Hillman, Todd A.
Buchinsky, Farrel J.
Tomasz, Alexander
de Lencastre, Herminia
Sá-Leão, Raquel
Post, J. Christopher
Hu, Fen Z.
Ehrlich, Garth D.
Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone
title Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone
title_full Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone
title_fullStr Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone
title_full_unstemmed Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone
title_short Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone
title_sort differences in genotype and virulence among four multidrug-resistant streptococcus pneumoniae isolates belonging to the pmen1 clone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242761/
https://www.ncbi.nlm.nih.gov/pubmed/22205975
http://dx.doi.org/10.1371/journal.pone.0028850
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