Cargando…

Kinetic Characterization of Single Strand Break Ligation in Duplex DNA by T4 DNA Ligase

T4 DNA ligase catalyzes phosphodiester bond formation between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA in three steps: 1) enzyme-adenylylate formation by reaction with ATP; 2) adenylyl transfer to a 5′-phosphorylated polynucleotide to generate adenylylated DNA; and 3) phosphodie...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lohman, Gregory J. S., Chen, Lixin, Evans, Thomas C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2011
Materias:	Enzymology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243518/ https://www.ncbi.nlm.nih.gov/pubmed/22027837 http://dx.doi.org/10.1074/jbc.M111.284992

Ejemplares similares

Structural Basis for the Inhibition of Human Alkyladenine DNA Glycosylase (AAG) by 3,N(4)-Ethenocytosine-containing DNA
por: Lingaraju, Gondichatnahalli M., et al.
Publicado: (2011)

Mechanism of Thiosulfate Oxidation in the SoxA Family of Cysteine-ligated Cytochromes
por: Grabarczyk, Daniel B., et al.
Publicado: (2015)

Characterization of the Human SNM1A and SNM1B/Apollo DNA Repair Exonucleases
por: Sengerová, Blanka, et al.
Publicado: (2012)

Catalysis of non-canonical protein ubiquitylation by the ARIH1 ubiquitin ligase
por: Purser, Nicholas, et al.
Publicado: (2023)

Pre-steady-state Kinetic Analysis of a Family D DNA Polymerase from Thermococcus sp. 9°N Reveals Mechanisms for Archaeal Genomic Replication and Maintenance
por: Schermerhorn, Kelly M., et al.
Publicado: (2015)

The Inhibitory Effect of Non-Substrate and Substrate DNA on the Ligation and Self-Adenylylation Reactions Catalyzed by T4 DNA Ligase
por: Bauer, Robert J., et al.
Publicado: (2016)

Efficient DNA ligation in DNA–RNA hybrid helices by Chlorella virus DNA ligase
por: Lohman, Gregory J. S., et al.
Publicado: (2014)

Heme ligation and redox chemistry in two bacterial thiosulfate dehydrogenase (TsdA) enzymes
por: Jenner, Leon P., et al.
Publicado: (2019)

Comparative Kinetic and Functional Characterization of the Motor Domains of Human Nonmuscle Myosin-2C Isoforms
por: Heissler, Sarah M., et al.
Publicado: (2011)

Characterization of the Elongation Complex of Dengue Virus RNA Polymerase: ASSEMBLY, KINETICS OF NUCLEOTIDE INCORPORATION, AND FIDELITY
por: Jin, Zhinan, et al.
Publicado: (2011)

Structural analyses of Arabidopsis thaliana legumain γ reveal differential recognition and processing of proteolysis and ligation substrates
por: Zauner, Florian B., et al.
Publicado: (2018)

Analysis of ubiquitin recognition by the HECT ligase E6AP provides insight into its linkage specificity
por: Ries, Lena K., et al.
Publicado: (2019)

Mechanical Stability and Fibrinolytic Resistance of Clots Containing Fibrin, DNA, and Histones
por: Longstaff, Colin, et al.
Publicado: (2013)

The Naphthoquinone Diospyrin Is an Inhibitor of DNA Gyrase with a Novel Mechanism of Action
por: Karkare, Shantanu, et al.
Publicado: (2013)

Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase
por: Lohman, G. J., et al.
Publicado: (2014)

The Steric Gate of DNA Polymerase ι Regulates Ribonucleotide Incorporation and Deoxyribonucleotide Fidelity
por: Donigan, Katherine A., et al.
Publicado: (2014)

Double-stranded RNA-dependent ATPase DRH-3: INSIGHT INTO ITS ROLE IN RNA SILENCING IN CAENORHABDITIS ELEGANS
por: Matranga, Christian, et al.
Publicado: (2010)

Single-stranded DNA ligation and XLF-stimulated incompatible DNA end ligation by the XRCC4-DNA ligase IV complex: influence of terminal DNA sequence
por: Gu, Jiafeng, et al.
Publicado: (2007)

Faster Protein Splicing with the Nostoc punctiforme DnaE Intein Using Non-native Extein Residues
por: Cheriyan, Manoj, et al.
Publicado: (2013)

The Roles of Family B and D DNA Polymerases in Thermococcus Species 9°N Okazaki Fragment Maturation
por: Greenough, Lucia, et al.
Publicado: (2015)

Dissecting the Catalytic Mechanism of Trypanosoma brucei Trypanothione Synthetase by Kinetic Analysis and Computational Modeling
por: Leroux, Alejandro E., et al.
Publicado: (2013)

Kinetic Investigations of the Role of Factor Inhibiting Hypoxia-inducible Factor (FIH) as an Oxygen Sensor
por: Tarhonskaya, Hanna, et al.
Publicado: (2015)

The plant cysteine oxidases from Arabidopsis thaliana are kinetically tailored to act as oxygen sensors
por: White, Mark D., et al.
Publicado: (2018)

Novosphingobium aromaticivorans uses a Nu-class glutathione S-transferase as a glutathione lyase in breaking the β-aryl ether bond of lignin
por: Kontur, Wayne S., et al.
Publicado: (2018)

A heterodimeric glutathione S-transferase that stereospecifically breaks lignin's β(R)-aryl ether bond reveals the diversity of bacterial β-etherases
por: Kontur, Wayne S., et al.
Publicado: (2019)

DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair
por: Abdou, Ismail, et al.
Publicado: (2015)

ATPase Mechanism of the 5′-3′ DNA Helicase, RecD2: EVIDENCE FOR A PRE-HYDROLYSIS CONFORMATION CHANGE
por: Toseland, Christopher P., et al.
Publicado: (2013)

SIRT1 Activation by Small Molecules: KINETIC AND BIOPHYSICAL EVIDENCE FOR DIRECT INTERACTION OF ENZYME AND ACTIVATOR
por: Dai, Han, et al.
Publicado: (2010)

Coupled chemistry kinetics demonstrate the utility of functionalized Sup35 amyloid nanofibrils in biocatalytic cascades
por: Schmuck, Benjamin, et al.
Publicado: (2019)

The Superfast Human Extraocular Myosin Is Kinetically Distinct from the Fast Skeletal IIa, IIb, and IId Isoforms
por: Bloemink, Marieke J., et al.
Publicado: (2013)

Assembly, Purification, and Pre-steady-state Kinetic Analysis of Active RNA-dependent RNA Polymerase Elongation Complex
por: Jin, Zhinan, et al.
Publicado: (2012)

Structural and Kinetic Studies of the Human Nudix Hydrolase MTH1 Reveal the Mechanism for Its Broad Substrate Specificity
por: Waz, Shaimaa, et al.
Publicado: (2017)

Pre-steady-state Kinetics Reveal the Substrate Specificity and Mechanism of Halide Oxidation of Truncated Human Peroxidasin 1
por: Paumann-Page, Martina, et al.
Publicado: (2017)

Kinetic parameters of human aspartate/asparagine–β-hydroxylase suggest that it has a possible function in oxygen sensing
por: Brewitz, Lennart, et al.
Publicado: (2020)

Structure and kinetics of indole-3-glycerol phosphate synthase from Pseudomonas aeruginosa: Decarboxylation is not essential for indole formation
por: Söderholm, Annika, et al.
Publicado: (2020)

Demonstration of Lignin-to-Peroxidase Direct Electron Transfer: A TRANSIENT-STATE KINETICS, DIRECTED MUTAGENESIS, EPR, AND NMR STUDY
por: Sáez-Jiménez, Verónica, et al.
Publicado: (2015)

Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates
por: Egger, Sigrid, et al.
Publicado: (2012)

A Single Residue Switch for Mg(2+)-dependent Inhibition Characterizes Plant Class II Diterpene Cyclases from Primary and Secondary Metabolism
por: Mann, Francis M., et al.
Publicado: (2010)

Roles of Phosphate Recognition in Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase (IPK1) Substrate Binding and Activation
por: Gosein, Varin, et al.
Publicado: (2013)

Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation
por: Hemu, Xinya, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_kruftlu41fmdo2ns8v24a7mfnp