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The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline

In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters, which are gene...

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Autores principales: Pane, Attilio, Jiang, Peng, Zhao, Dorothy Yanling, Singh, Mona, Schüpbach, Trudi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Molecular Biology Organization 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243597/
https://www.ncbi.nlm.nih.gov/pubmed/21952049
http://dx.doi.org/10.1038/emboj.2011.334
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author Pane, Attilio
Jiang, Peng
Zhao, Dorothy Yanling
Singh, Mona
Schüpbach, Trudi
author_facet Pane, Attilio
Jiang, Peng
Zhao, Dorothy Yanling
Singh, Mona
Schüpbach, Trudi
author_sort Pane, Attilio
collection PubMed
description In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters, which are generally embedded in heterochromatic regions. The molecular mechanisms and the factors that govern their expression are largely unknown. Here, we show that Cutoff (Cuff), a Drosophila protein related to the yeast transcription termination factor Rai1, is essential for piRNA production in germline tissues. Cuff accumulates at centromeric/pericentromeric positions in germ-cell nuclei and strongly colocalizes with the major heterochromatic domains. Remarkably, we show that Cuff is enriched at the dual-strand piRNA cluster 1/42AB and is likely to be involved in regulation of transcript levels of similar loci dispersed in the genome. Consistent with this observation, Cuff physically interacts with the Heterochromatin Protein 1 (HP1) variant Rhino (Rhi). Our results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila.
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spelling pubmed-32435972011-12-23 The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline Pane, Attilio Jiang, Peng Zhao, Dorothy Yanling Singh, Mona Schüpbach, Trudi EMBO J Article In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters, which are generally embedded in heterochromatic regions. The molecular mechanisms and the factors that govern their expression are largely unknown. Here, we show that Cutoff (Cuff), a Drosophila protein related to the yeast transcription termination factor Rai1, is essential for piRNA production in germline tissues. Cuff accumulates at centromeric/pericentromeric positions in germ-cell nuclei and strongly colocalizes with the major heterochromatic domains. Remarkably, we show that Cuff is enriched at the dual-strand piRNA cluster 1/42AB and is likely to be involved in regulation of transcript levels of similar loci dispersed in the genome. Consistent with this observation, Cuff physically interacts with the Heterochromatin Protein 1 (HP1) variant Rhino (Rhi). Our results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila. European Molecular Biology Organization 2011-11-16 2011-09-27 /pmc/articles/PMC3243597/ /pubmed/21952049 http://dx.doi.org/10.1038/emboj.2011.334 Text en Copyright © 2011, European Molecular Biology Organization https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
spellingShingle Article
Pane, Attilio
Jiang, Peng
Zhao, Dorothy Yanling
Singh, Mona
Schüpbach, Trudi
The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
title The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
title_full The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
title_fullStr The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
title_full_unstemmed The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
title_short The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
title_sort cutoff protein regulates pirna cluster expression and pirna production in the drosophila germline
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243597/
https://www.ncbi.nlm.nih.gov/pubmed/21952049
http://dx.doi.org/10.1038/emboj.2011.334
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