Cargando…
The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters, which are gene...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243597/ https://www.ncbi.nlm.nih.gov/pubmed/21952049 http://dx.doi.org/10.1038/emboj.2011.334 |
_version_ | 1782219679682527232 |
---|---|
author | Pane, Attilio Jiang, Peng Zhao, Dorothy Yanling Singh, Mona Schüpbach, Trudi |
author_facet | Pane, Attilio Jiang, Peng Zhao, Dorothy Yanling Singh, Mona Schüpbach, Trudi |
author_sort | Pane, Attilio |
collection | PubMed |
description | In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters, which are generally embedded in heterochromatic regions. The molecular mechanisms and the factors that govern their expression are largely unknown. Here, we show that Cutoff (Cuff), a Drosophila protein related to the yeast transcription termination factor Rai1, is essential for piRNA production in germline tissues. Cuff accumulates at centromeric/pericentromeric positions in germ-cell nuclei and strongly colocalizes with the major heterochromatic domains. Remarkably, we show that Cuff is enriched at the dual-strand piRNA cluster 1/42AB and is likely to be involved in regulation of transcript levels of similar loci dispersed in the genome. Consistent with this observation, Cuff physically interacts with the Heterochromatin Protein 1 (HP1) variant Rhino (Rhi). Our results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila. |
format | Online Article Text |
id | pubmed-3243597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-32435972011-12-23 The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline Pane, Attilio Jiang, Peng Zhao, Dorothy Yanling Singh, Mona Schüpbach, Trudi EMBO J Article In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters, which are generally embedded in heterochromatic regions. The molecular mechanisms and the factors that govern their expression are largely unknown. Here, we show that Cutoff (Cuff), a Drosophila protein related to the yeast transcription termination factor Rai1, is essential for piRNA production in germline tissues. Cuff accumulates at centromeric/pericentromeric positions in germ-cell nuclei and strongly colocalizes with the major heterochromatic domains. Remarkably, we show that Cuff is enriched at the dual-strand piRNA cluster 1/42AB and is likely to be involved in regulation of transcript levels of similar loci dispersed in the genome. Consistent with this observation, Cuff physically interacts with the Heterochromatin Protein 1 (HP1) variant Rhino (Rhi). Our results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila. European Molecular Biology Organization 2011-11-16 2011-09-27 /pmc/articles/PMC3243597/ /pubmed/21952049 http://dx.doi.org/10.1038/emboj.2011.334 Text en Copyright © 2011, European Molecular Biology Organization https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Article Pane, Attilio Jiang, Peng Zhao, Dorothy Yanling Singh, Mona Schüpbach, Trudi The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline |
title | The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline |
title_full | The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline |
title_fullStr | The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline |
title_full_unstemmed | The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline |
title_short | The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline |
title_sort | cutoff protein regulates pirna cluster expression and pirna production in the drosophila germline |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243597/ https://www.ncbi.nlm.nih.gov/pubmed/21952049 http://dx.doi.org/10.1038/emboj.2011.334 |
work_keys_str_mv | AT paneattilio thecutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT jiangpeng thecutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT zhaodorothyyanling thecutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT singhmona thecutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT schupbachtrudi thecutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT paneattilio cutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT jiangpeng cutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT zhaodorothyyanling cutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT singhmona cutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline AT schupbachtrudi cutoffproteinregulatespirnaclusterexpressionandpirnaproductioninthedrosophilagermline |