Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain

The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrop...

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Autores principales: Halder, Partho, Chen, Yi-chun, Brauckhoff, Janine, Hofbauer, Alois, Dabauvalle, Marie-Christine, Lewandrowski, Urs, Winkler, Christiane, Sickmann, Albert, Buchner, Erich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244249/
https://www.ncbi.nlm.nih.gov/pubmed/22206011
http://dx.doi.org/10.1371/journal.pone.0029352
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author Halder, Partho
Chen, Yi-chun
Brauckhoff, Janine
Hofbauer, Alois
Dabauvalle, Marie-Christine
Lewandrowski, Urs
Winkler, Christiane
Sickmann, Albert
Buchner, Erich
author_facet Halder, Partho
Chen, Yi-chun
Brauckhoff, Janine
Hofbauer, Alois
Dabauvalle, Marie-Christine
Lewandrowski, Urs
Winkler, Christiane
Sickmann, Albert
Buchner, Erich
author_sort Halder, Partho
collection PubMed
description The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.
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spelling pubmed-32442492011-12-28 Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain Halder, Partho Chen, Yi-chun Brauckhoff, Janine Hofbauer, Alois Dabauvalle, Marie-Christine Lewandrowski, Urs Winkler, Christiane Sickmann, Albert Buchner, Erich PLoS One Research Article The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies. Public Library of Science 2011-12-19 /pmc/articles/PMC3244249/ /pubmed/22206011 http://dx.doi.org/10.1371/journal.pone.0029352 Text en Halder et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Halder, Partho
Chen, Yi-chun
Brauckhoff, Janine
Hofbauer, Alois
Dabauvalle, Marie-Christine
Lewandrowski, Urs
Winkler, Christiane
Sickmann, Albert
Buchner, Erich
Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain
title Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain
title_full Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain
title_fullStr Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain
title_full_unstemmed Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain
title_short Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain
title_sort identification of eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the wuerzburg hybridoma library against drosophila brain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244249/
https://www.ncbi.nlm.nih.gov/pubmed/22206011
http://dx.doi.org/10.1371/journal.pone.0029352
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