Cargando…
Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer
The potential application of multiplexed quantum dot labeling (MQDL) for cancer detection and prognosis and monitoring therapeutic responses has attracted the interests of bioengineers, pathologists and cancer biologists. Many published studies claim that MQDL is effective for cancer biomarker detec...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244400/ https://www.ncbi.nlm.nih.gov/pubmed/22205960 http://dx.doi.org/10.1371/journal.pone.0028670 |
_version_ | 1782219723558092800 |
---|---|
author | Hu, Peizhen Chu, Gina C.-Y. Zhu, Guodong Yang, Hua Luthringer, Daniel Prins, Gail Habib, Fouad Wang, Yuzhuo Wang, Ruoxiang Chung, Leland W. K. Zhau, Haiyen E. |
author_facet | Hu, Peizhen Chu, Gina C.-Y. Zhu, Guodong Yang, Hua Luthringer, Daniel Prins, Gail Habib, Fouad Wang, Yuzhuo Wang, Ruoxiang Chung, Leland W. K. Zhau, Haiyen E. |
author_sort | Hu, Peizhen |
collection | PubMed |
description | The potential application of multiplexed quantum dot labeling (MQDL) for cancer detection and prognosis and monitoring therapeutic responses has attracted the interests of bioengineers, pathologists and cancer biologists. Many published studies claim that MQDL is effective for cancer biomarker detection and useful in cancer diagnosis and prognosis, these studies have not been standardized against quantitative biochemical and molecular determinations. In the present study, we used a molecularly characterized human prostate cancer cell model exhibiting activated c-Met signaling with epithelial to mesenchymal transition (EMT) and lethal metastatic progression to bone and soft tissues as the gold standard, and compared the c-Met cell signaling network in this model, in clinical human prostate cancer tissue specimens and in a castration-resistant human prostate cancer xenograft model. We observed c-Met signaling network activation, manifested by increased phosphorylated c-Met in all three. The downstream survival signaling network was mediated by NF-κB and Mcl-1 and EMT was driven by receptor activator of NF-κB ligand (RANKL), at the single cell level in clinical prostate cancer specimens and the xenograft model. Results were confirmed by real-time RT-PCR and western blots in a human prostate cancer cell model. MQDL is a powerful tool for assessing biomarker expression and it offers molecular insights into cancer progression at both the cell and tissue level with high degree of sensitivity. |
format | Online Article Text |
id | pubmed-3244400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32444002011-12-28 Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer Hu, Peizhen Chu, Gina C.-Y. Zhu, Guodong Yang, Hua Luthringer, Daniel Prins, Gail Habib, Fouad Wang, Yuzhuo Wang, Ruoxiang Chung, Leland W. K. Zhau, Haiyen E. PLoS One Research Article The potential application of multiplexed quantum dot labeling (MQDL) for cancer detection and prognosis and monitoring therapeutic responses has attracted the interests of bioengineers, pathologists and cancer biologists. Many published studies claim that MQDL is effective for cancer biomarker detection and useful in cancer diagnosis and prognosis, these studies have not been standardized against quantitative biochemical and molecular determinations. In the present study, we used a molecularly characterized human prostate cancer cell model exhibiting activated c-Met signaling with epithelial to mesenchymal transition (EMT) and lethal metastatic progression to bone and soft tissues as the gold standard, and compared the c-Met cell signaling network in this model, in clinical human prostate cancer tissue specimens and in a castration-resistant human prostate cancer xenograft model. We observed c-Met signaling network activation, manifested by increased phosphorylated c-Met in all three. The downstream survival signaling network was mediated by NF-κB and Mcl-1 and EMT was driven by receptor activator of NF-κB ligand (RANKL), at the single cell level in clinical prostate cancer specimens and the xenograft model. Results were confirmed by real-time RT-PCR and western blots in a human prostate cancer cell model. MQDL is a powerful tool for assessing biomarker expression and it offers molecular insights into cancer progression at both the cell and tissue level with high degree of sensitivity. Public Library of Science 2011-12-21 /pmc/articles/PMC3244400/ /pubmed/22205960 http://dx.doi.org/10.1371/journal.pone.0028670 Text en Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hu, Peizhen Chu, Gina C.-Y. Zhu, Guodong Yang, Hua Luthringer, Daniel Prins, Gail Habib, Fouad Wang, Yuzhuo Wang, Ruoxiang Chung, Leland W. K. Zhau, Haiyen E. Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer |
title | Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer |
title_full | Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer |
title_fullStr | Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer |
title_full_unstemmed | Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer |
title_short | Multiplexed Quantum Dot Labeling of Activated c-Met Signaling in Castration-Resistant Human Prostate Cancer |
title_sort | multiplexed quantum dot labeling of activated c-met signaling in castration-resistant human prostate cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244400/ https://www.ncbi.nlm.nih.gov/pubmed/22205960 http://dx.doi.org/10.1371/journal.pone.0028670 |
work_keys_str_mv | AT hupeizhen multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT chuginacy multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT zhuguodong multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT yanghua multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT luthringerdaniel multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT prinsgail multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT habibfouad multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT wangyuzhuo multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT wangruoxiang multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT chunglelandwk multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer AT zhauhaiyene multiplexedquantumdotlabelingofactivatedcmetsignalingincastrationresistanthumanprostatecancer |