A Viral Discovery Methodology for Clinical Biopsy Samples Utilising Massively Parallel Next Generation Sequencing

Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral conti...

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Detalles Bibliográficos
Autores principales: Daly, Gordon M., Bexfield, Nick, Heaney, Judith, Stubbs, Sam, Mayer, Antonia P., Palser, Anne, Kellam, Paul, Drou, Nizar, Caccamo, Mario, Tiley, Laurence, Alexander, Graeme J. M., Bernal, William, Heeney, Jonathan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244418/
https://www.ncbi.nlm.nih.gov/pubmed/22216131
http://dx.doi.org/10.1371/journal.pone.0028879
Descripción
Sumario:Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.

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