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Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction
PURPOSE: Recently, arginine vasopressin (AVP) has been revealed to have diverse functional roles in nervous tissues beyond that of a vasoconstrictor. Several previous studies have indicated the existence of AVP in the retina, but the source of AVP has not been determined. The objective of the presen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244476/ https://www.ncbi.nlm.nih.gov/pubmed/22194651 |
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author | Moritoh, Satoru Sato, Kaori Okada, Yasunobu Koizumi, Amane |
author_facet | Moritoh, Satoru Sato, Kaori Okada, Yasunobu Koizumi, Amane |
author_sort | Moritoh, Satoru |
collection | PubMed |
description | PURPOSE: Recently, arginine vasopressin (AVP) has been revealed to have diverse functional roles in nervous tissues beyond that of a vasoconstrictor. Several previous studies have indicated the existence of AVP in the retina, but the source of AVP has not been determined. The objective of the present study was to address the question of whether retinal cells have the ability to synthesize endogenous AVP to act in a paracrine or autocrine manner. METHODS: We used AVP-eGFP transgenic rats to find endogenous AVP-positive cells in the retina by immunohistochemistry with an AVP antibody and a GFP antibody. We also examined AVP mRNA and AVP receptor genes by reverse transcriptase (RT)–PCR of dissociated GFP-positive cells and whole retinas. RESULTS: Endogenous AVP-positive cells were found in the ganglion cell layer and inner nuclear layer of the retina of AVP-eGFP transgenic rats by immunohistochemistry. As indicated by the results of RT–PCR of dissociated GFP-positive cells, these cells have the ability to synthesize endogenous AVP, as well as transgenic AVP-eGFP. In addition, the V1a and V1b AVP receptors were found in the wild-type rat retina by whole retina RT–PCR, but the V2 receptor was not detectable. In dissociated AVP-eGFP-positive cells, no AVP receptor was detected by RT–PCR. Moreover, AVP secretion was not detected by stimulation with a high potassium (50 mM) solution. CONCLUSIONS: In the rat retina, we found retinal cells that have the ability to synthesize endogenous AVP, and that the retina possesses V1a and V1b AVP receptors. Taken together, these results suggest that the retina has an intrinsic AVP-synthesizing and -receiving mechanism that can operate in a paracrine manner via V1a and V1b receptors. |
format | Online Article Text |
id | pubmed-3244476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-32444762011-12-22 Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction Moritoh, Satoru Sato, Kaori Okada, Yasunobu Koizumi, Amane Mol Vis Research Article PURPOSE: Recently, arginine vasopressin (AVP) has been revealed to have diverse functional roles in nervous tissues beyond that of a vasoconstrictor. Several previous studies have indicated the existence of AVP in the retina, but the source of AVP has not been determined. The objective of the present study was to address the question of whether retinal cells have the ability to synthesize endogenous AVP to act in a paracrine or autocrine manner. METHODS: We used AVP-eGFP transgenic rats to find endogenous AVP-positive cells in the retina by immunohistochemistry with an AVP antibody and a GFP antibody. We also examined AVP mRNA and AVP receptor genes by reverse transcriptase (RT)–PCR of dissociated GFP-positive cells and whole retinas. RESULTS: Endogenous AVP-positive cells were found in the ganglion cell layer and inner nuclear layer of the retina of AVP-eGFP transgenic rats by immunohistochemistry. As indicated by the results of RT–PCR of dissociated GFP-positive cells, these cells have the ability to synthesize endogenous AVP, as well as transgenic AVP-eGFP. In addition, the V1a and V1b AVP receptors were found in the wild-type rat retina by whole retina RT–PCR, but the V2 receptor was not detectable. In dissociated AVP-eGFP-positive cells, no AVP receptor was detected by RT–PCR. Moreover, AVP secretion was not detected by stimulation with a high potassium (50 mM) solution. CONCLUSIONS: In the rat retina, we found retinal cells that have the ability to synthesize endogenous AVP, and that the retina possesses V1a and V1b AVP receptors. Taken together, these results suggest that the retina has an intrinsic AVP-synthesizing and -receiving mechanism that can operate in a paracrine manner via V1a and V1b receptors. Molecular Vision 2011-12-15 /pmc/articles/PMC3244476/ /pubmed/22194651 Text en Copyright © 2011 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Moritoh, Satoru Sato, Kaori Okada, Yasunobu Koizumi, Amane Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction |
title | Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction |
title_full | Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction |
title_fullStr | Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction |
title_full_unstemmed | Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction |
title_short | Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction |
title_sort | endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–egfp transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244476/ https://www.ncbi.nlm.nih.gov/pubmed/22194651 |
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