Single cell transcriptomics of neighboring hyphae of Aspergillus niger

Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger gen...

Descripción completa

Detalles Bibliográficos
Autores principales: de Bekker, Charissa, Bruning, Oskar, Jonker, Martijs J, Breit, Timo M, Wösten, Han AB
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245611/
https://www.ncbi.nlm.nih.gov/pubmed/21816052
http://dx.doi.org/10.1186/gb-2011-12-8-r71
_version_ 1782219884904579072
author de Bekker, Charissa
Bruning, Oskar
Jonker, Martijs J
Breit, Timo M
Wösten, Han AB
author_facet de Bekker, Charissa
Bruning, Oskar
Jonker, Martijs J
Breit, Timo M
Wösten, Han AB
author_sort de Bekker, Charissa
collection PubMed
description Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.
format Online
Article
Text
id pubmed-3245611
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-32456112011-12-24 Single cell transcriptomics of neighboring hyphae of Aspergillus niger de Bekker, Charissa Bruning, Oskar Jonker, Martijs J Breit, Timo M Wösten, Han AB Genome Biol Method Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories. BioMed Central 2011 2011-08-04 /pmc/articles/PMC3245611/ /pubmed/21816052 http://dx.doi.org/10.1186/gb-2011-12-8-r71 Text en Copyright ©2011 de Bekker et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
de Bekker, Charissa
Bruning, Oskar
Jonker, Martijs J
Breit, Timo M
Wösten, Han AB
Single cell transcriptomics of neighboring hyphae of Aspergillus niger
title Single cell transcriptomics of neighboring hyphae of Aspergillus niger
title_full Single cell transcriptomics of neighboring hyphae of Aspergillus niger
title_fullStr Single cell transcriptomics of neighboring hyphae of Aspergillus niger
title_full_unstemmed Single cell transcriptomics of neighboring hyphae of Aspergillus niger
title_short Single cell transcriptomics of neighboring hyphae of Aspergillus niger
title_sort single cell transcriptomics of neighboring hyphae of aspergillus niger
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245611/
https://www.ncbi.nlm.nih.gov/pubmed/21816052
http://dx.doi.org/10.1186/gb-2011-12-8-r71
work_keys_str_mv AT debekkercharissa singlecelltranscriptomicsofneighboringhyphaeofaspergillusniger
AT bruningoskar singlecelltranscriptomicsofneighboringhyphaeofaspergillusniger
AT jonkermartijsj singlecelltranscriptomicsofneighboringhyphaeofaspergillusniger
AT breittimom singlecelltranscriptomicsofneighboringhyphaeofaspergillusniger
AT wostenhanab singlecelltranscriptomicsofneighboringhyphaeofaspergillusniger

Ejemplares similares