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Hybrid selection for sequencing pathogen genomes from clinical samples

We have adapted a solution hybrid selection protocol to enrich pathogen DNA in clinical samples dominated by human genetic material. Using mock mixtures of human and Plasmodium falciparum malaria parasite DNA as well as clinical samples from infected patients, we demonstrate an average of approximat...

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Detalles Bibliográficos
Autores principales: Melnikov, Alexandre, Galinsky, Kevin, Rogov, Peter, Fennell, Timothy, Van Tyne, Daria, Russ, Carsten, Daniels, Rachel, Barnes, Kayla G, Bochicchio, James, Ndiaye, Daouda, Sene, Papa D, Wirth, Dyann F, Nusbaum, Chad, Volkman, Sarah K, Birren, Bruce W, Gnirke, Andreas, Neafsey, Daniel E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245613/
https://www.ncbi.nlm.nih.gov/pubmed/21835008
http://dx.doi.org/10.1186/gb-2011-12-8-r73
Descripción
Sumario:We have adapted a solution hybrid selection protocol to enrich pathogen DNA in clinical samples dominated by human genetic material. Using mock mixtures of human and Plasmodium falciparum malaria parasite DNA as well as clinical samples from infected patients, we demonstrate an average of approximately 40-fold enrichment of parasite DNA after hybrid selection. This approach will enable efficient genome sequencing of pathogens from clinical samples, as well as sequencing of endosymbiotic organisms such as Wolbachia that live inside diverse metazoan phyla.