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Hybrid selection for sequencing pathogen genomes from clinical samples
We have adapted a solution hybrid selection protocol to enrich pathogen DNA in clinical samples dominated by human genetic material. Using mock mixtures of human and Plasmodium falciparum malaria parasite DNA as well as clinical samples from infected patients, we demonstrate an average of approximat...
Autores principales: | Melnikov, Alexandre, Galinsky, Kevin, Rogov, Peter, Fennell, Timothy, Van Tyne, Daria, Russ, Carsten, Daniels, Rachel, Barnes, Kayla G, Bochicchio, James, Ndiaye, Daouda, Sene, Papa D, Wirth, Dyann F, Nusbaum, Chad, Volkman, Sarah K, Birren, Bruce W, Gnirke, Andreas, Neafsey, Daniel E |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245613/ https://www.ncbi.nlm.nih.gov/pubmed/21835008 http://dx.doi.org/10.1186/gb-2011-12-8-r73 |
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