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The MORPHEUS protein crystallization screen

A 96-condition initial screen for protein crystallization, called MORPHEUS, has been developed at the MRC Laboratory of Molecular Biology, Cambridge, England (MRC-LMB). The concept integrates several innovative approaches, such as chemically compatible mixes of potential ligands, new buffer systems...

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Autor principal: Gorrec, Fabrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246824/
https://www.ncbi.nlm.nih.gov/pubmed/22477774
http://dx.doi.org/10.1107/S0021889809042022
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author Gorrec, Fabrice
author_facet Gorrec, Fabrice
author_sort Gorrec, Fabrice
collection PubMed
description A 96-condition initial screen for protein crystallization, called MORPHEUS, has been developed at the MRC Laboratory of Molecular Biology, Cambridge, England (MRC-LMB). The concept integrates several innovative approaches, such as chemically compatible mixes of potential ligands, new buffer systems and precipitant mixes that also act as cryoprotectants. Instead of gathering a set of crystallization conditions that have already been successful, a selection of molecules frequently observed in the Protein Data Bank (PDB) to co-crystallize with proteins has been made. These have been put together in mixes of similar chemical behaviour and structure, and combined with buffers and precipitant mixes that were also derived from PDB searches, to build the screen de novo. Observations made at the MRC-LMB and many practical aspects were also taken into account when formulating the screen. The resulting screen is easy to use, comprehensive yet small, and has already yielded a list of crystallization hits using both known and novel samples. As an indicator of success, the screen has now become one of the standard screens used routinely at the MRC-LMB when searching initial crystallization conditions for biological macromolecules.
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spelling pubmed-32468242012-01-06 The MORPHEUS protein crystallization screen Gorrec, Fabrice J Appl Crystallogr Research Papers A 96-condition initial screen for protein crystallization, called MORPHEUS, has been developed at the MRC Laboratory of Molecular Biology, Cambridge, England (MRC-LMB). The concept integrates several innovative approaches, such as chemically compatible mixes of potential ligands, new buffer systems and precipitant mixes that also act as cryoprotectants. Instead of gathering a set of crystallization conditions that have already been successful, a selection of molecules frequently observed in the Protein Data Bank (PDB) to co-crystallize with proteins has been made. These have been put together in mixes of similar chemical behaviour and structure, and combined with buffers and precipitant mixes that were also derived from PDB searches, to build the screen de novo. Observations made at the MRC-LMB and many practical aspects were also taken into account when formulating the screen. The resulting screen is easy to use, comprehensive yet small, and has already yielded a list of crystallization hits using both known and novel samples. As an indicator of success, the screen has now become one of the standard screens used routinely at the MRC-LMB when searching initial crystallization conditions for biological macromolecules. International Union of Crystallography 2009-11-07 /pmc/articles/PMC3246824/ /pubmed/22477774 http://dx.doi.org/10.1107/S0021889809042022 Text en © Fabrice Gorrec 2009 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Gorrec, Fabrice
The MORPHEUS protein crystallization screen
title The MORPHEUS protein crystallization screen
title_full The MORPHEUS protein crystallization screen
title_fullStr The MORPHEUS protein crystallization screen
title_full_unstemmed The MORPHEUS protein crystallization screen
title_short The MORPHEUS protein crystallization screen
title_sort morpheus protein crystallization screen
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246824/
https://www.ncbi.nlm.nih.gov/pubmed/22477774
http://dx.doi.org/10.1107/S0021889809042022
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