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Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line
BACKGROUND: Hepatitis C virus (HCV) Core protein regulates multiple signaling pathways and alters cellular genes expression responsible for HCV induced pathogenesis leading to hepatocellular carcinoma (HCC). Prevalence of HCV genotype 3a associated HCC is higher in Pakistan as compare to the rest of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247135/ https://www.ncbi.nlm.nih.gov/pubmed/22108100 http://dx.doi.org/10.1186/1743-422X-8-522 |
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author | Jahan, Shah Khaliq, Saba Siddiqi, Muhammad Hassan Ijaz, Bushra Ahmad, Waqar Ashfaq, Usman A Hassan, Sajida |
author_facet | Jahan, Shah Khaliq, Saba Siddiqi, Muhammad Hassan Ijaz, Bushra Ahmad, Waqar Ashfaq, Usman A Hassan, Sajida |
author_sort | Jahan, Shah |
collection | PubMed |
description | BACKGROUND: Hepatitis C virus (HCV) Core protein regulates multiple signaling pathways and alters cellular genes expression responsible for HCV induced pathogenesis leading to hepatocellular carcinoma (HCC). Prevalence of HCV genotype 3a associated HCC is higher in Pakistan as compare to the rest of world; however the molecular mechanism behind this is still unclear. This study has been designed to evaluate the effect of HCV core 3a on apoptosis and cell proliferation which are involved in HCC METHODOLOGY: We examined the in vitro effect of HCV Core protein of genotype 3a and 1a on cellular genes involved in apoptosis by Real time PCR in liver cell line (Huh-7). We analyzed the effect of HCV core of genotype 1a and 3a on cell proliferation by MTT assay and on phosphrylation of Akt by western blotting in Huh-7 cells. RESULTS: The HCV 3a Core down regulates the gene expression of Caspases (3, 8, 9 and 10), Cyto C and p53 which are involved in apoptosis. Moreover, HCV 3a Core gene showed stronger effect in regulating protein level of p-Akt as compared to HCV 1a Core accompanied by enhanced cell proliferation in Huh-7 cell line. CONCLUSION: From the current study it has been concluded that reduced expression of cellular genes involved in apoptosis, increased p-Akt (cell survival gene) and enhanced cell proliferation in response to HCV 3a core confirms anti apoptotic effect of HCV 3a Core gene in Huh-7 that may lead to HCC. |
format | Online Article Text |
id | pubmed-3247135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32471352011-12-29 Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line Jahan, Shah Khaliq, Saba Siddiqi, Muhammad Hassan Ijaz, Bushra Ahmad, Waqar Ashfaq, Usman A Hassan, Sajida Virol J Research BACKGROUND: Hepatitis C virus (HCV) Core protein regulates multiple signaling pathways and alters cellular genes expression responsible for HCV induced pathogenesis leading to hepatocellular carcinoma (HCC). Prevalence of HCV genotype 3a associated HCC is higher in Pakistan as compare to the rest of world; however the molecular mechanism behind this is still unclear. This study has been designed to evaluate the effect of HCV core 3a on apoptosis and cell proliferation which are involved in HCC METHODOLOGY: We examined the in vitro effect of HCV Core protein of genotype 3a and 1a on cellular genes involved in apoptosis by Real time PCR in liver cell line (Huh-7). We analyzed the effect of HCV core of genotype 1a and 3a on cell proliferation by MTT assay and on phosphrylation of Akt by western blotting in Huh-7 cells. RESULTS: The HCV 3a Core down regulates the gene expression of Caspases (3, 8, 9 and 10), Cyto C and p53 which are involved in apoptosis. Moreover, HCV 3a Core gene showed stronger effect in regulating protein level of p-Akt as compared to HCV 1a Core accompanied by enhanced cell proliferation in Huh-7 cell line. CONCLUSION: From the current study it has been concluded that reduced expression of cellular genes involved in apoptosis, increased p-Akt (cell survival gene) and enhanced cell proliferation in response to HCV 3a core confirms anti apoptotic effect of HCV 3a Core gene in Huh-7 that may lead to HCC. BioMed Central 2011-11-23 /pmc/articles/PMC3247135/ /pubmed/22108100 http://dx.doi.org/10.1186/1743-422X-8-522 Text en Copyright ©2011 Jahan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Jahan, Shah Khaliq, Saba Siddiqi, Muhammad Hassan Ijaz, Bushra Ahmad, Waqar Ashfaq, Usman A Hassan, Sajida Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line |
title | Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line |
title_full | Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line |
title_fullStr | Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line |
title_full_unstemmed | Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line |
title_short | Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line |
title_sort | anti-apoptotic effect of hcv core gene of genotype 3a in huh-7 cell line |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247135/ https://www.ncbi.nlm.nih.gov/pubmed/22108100 http://dx.doi.org/10.1186/1743-422X-8-522 |
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