Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies

Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified...

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Detalles Bibliográficos
Autores principales: Cornett, Jonathan C., Landrette, Sean F., Xu, Tian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247282/
https://www.ncbi.nlm.nih.gov/pubmed/22216292
http://dx.doi.org/10.1371/journal.pone.0029486
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author Cornett, Jonathan C.
Landrette, Sean F.
Xu, Tian
author_facet Cornett, Jonathan C.
Landrette, Sean F.
Xu, Tian
author_sort Cornett, Jonathan C.
collection PubMed
description Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified and that fluorescence intensity in the eyes can be used to distinguish heterozygous from homozygous mice. Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction.
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spelling pubmed-32472822012-01-03 Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies Cornett, Jonathan C. Landrette, Sean F. Xu, Tian PLoS One Research Article Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified and that fluorescence intensity in the eyes can be used to distinguish heterozygous from homozygous mice. Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction. Public Library of Science 2011-12-28 /pmc/articles/PMC3247282/ /pubmed/22216292 http://dx.doi.org/10.1371/journal.pone.0029486 Text en Cornett et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cornett, Jonathan C.
Landrette, Sean F.
Xu, Tian
Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies
title Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies
title_full Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies
title_fullStr Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies
title_full_unstemmed Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies
title_short Characterization of Fluorescent Eye Markers for Mammalian Transgenic Studies
title_sort characterization of fluorescent eye markers for mammalian transgenic studies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247282/
https://www.ncbi.nlm.nih.gov/pubmed/22216292
http://dx.doi.org/10.1371/journal.pone.0029486
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