Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium

Immunosensors based on gold electrodes (electrochemical) or gold discs (optical) modified with 1,6-hexanedithiol, gold nanorods and Anti-His (C-term) monoclonal antibody F(ab’) fragment are described. The antigen detected by the sensing platform is a recombinant histidine-tagged silk proteinase inhi...

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Autores principales: Wąsowicz, Michal, Milner, Małgorzata, Radecka, Dorota, Grzelak, Krystyna, Radecka, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247714/
https://www.ncbi.nlm.nih.gov/pubmed/22219669
http://dx.doi.org/10.3390/s100605409
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author Wąsowicz, Michal
Milner, Małgorzata
Radecka, Dorota
Grzelak, Krystyna
Radecka, Hanna
author_facet Wąsowicz, Michal
Milner, Małgorzata
Radecka, Dorota
Grzelak, Krystyna
Radecka, Hanna
author_sort Wąsowicz, Michal
collection PubMed
description Immunosensors based on gold electrodes (electrochemical) or gold discs (optical) modified with 1,6-hexanedithiol, gold nanorods and Anti-His (C-term) monoclonal antibody F(ab’) fragment are described. The antigen detected by the sensing platform is a recombinant histidine-tagged silk proteinase inhibitor (rSPI2-His(6)). Electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) techniques were used as methods for detection of the antigen. This approach allows to detect the antigen protein in concentration of 10 pg per mL (0.13 pM) of culture medium. The immunosensor shows good reproducibility due to covalent immobilization of F(ab’) fragments to gold nanorods layer.
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spelling pubmed-32477142012-01-04 Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium Wąsowicz, Michal Milner, Małgorzata Radecka, Dorota Grzelak, Krystyna Radecka, Hanna Sensors (Basel) Article Immunosensors based on gold electrodes (electrochemical) or gold discs (optical) modified with 1,6-hexanedithiol, gold nanorods and Anti-His (C-term) monoclonal antibody F(ab’) fragment are described. The antigen detected by the sensing platform is a recombinant histidine-tagged silk proteinase inhibitor (rSPI2-His(6)). Electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) techniques were used as methods for detection of the antigen. This approach allows to detect the antigen protein in concentration of 10 pg per mL (0.13 pM) of culture medium. The immunosensor shows good reproducibility due to covalent immobilization of F(ab’) fragments to gold nanorods layer. Molecular Diversity Preservation International (MDPI) 2010-06-01 /pmc/articles/PMC3247714/ /pubmed/22219669 http://dx.doi.org/10.3390/s100605409 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wąsowicz, Michal
Milner, Małgorzata
Radecka, Dorota
Grzelak, Krystyna
Radecka, Hanna
Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium
title Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium
title_full Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium
title_fullStr Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium
title_full_unstemmed Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium
title_short Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium
title_sort immunosensor incorporating anti-his (c-term) igg f(ab’) fragments attached to gold nanorods for detection of his-tagged proteins in culture medium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247714/
https://www.ncbi.nlm.nih.gov/pubmed/22219669
http://dx.doi.org/10.3390/s100605409
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