Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor

A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized...

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Detalles Bibliográficos
Autores principales: Hsu, Shu-Yi, Bartling, Brandon, Wang, Christina, Shieu, Fuh-Sheng, Liu, Chung-Chiun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247730/
https://www.ncbi.nlm.nih.gov/pubmed/22219685
http://dx.doi.org/10.3390/s100605758
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author Hsu, Shu-Yi
Bartling, Brandon
Wang, Christina
Shieu, Fuh-Sheng
Liu, Chung-Chiun
author_facet Hsu, Shu-Yi
Bartling, Brandon
Wang, Christina
Shieu, Fuh-Sheng
Liu, Chung-Chiun
author_sort Hsu, Shu-Yi
collection PubMed
description A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.
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spelling pubmed-32477302012-01-04 Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor Hsu, Shu-Yi Bartling, Brandon Wang, Christina Shieu, Fuh-Sheng Liu, Chung-Chiun Sensors (Basel) Article A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media. Molecular Diversity Preservation International (MDPI) 2010-06-08 /pmc/articles/PMC3247730/ /pubmed/22219685 http://dx.doi.org/10.3390/s100605758 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Hsu, Shu-Yi
Bartling, Brandon
Wang, Christina
Shieu, Fuh-Sheng
Liu, Chung-Chiun
Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor
title Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor
title_full Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor
title_fullStr Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor
title_full_unstemmed Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor
title_short Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor
title_sort enzymatic determination of diglyceride using an iridium nano-particle based single use, disposable biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247730/
https://www.ncbi.nlm.nih.gov/pubmed/22219685
http://dx.doi.org/10.3390/s100605758
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