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Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors

We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution th...

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Autores principales: Takamatsu, Seiichi, Takano, Hisanori, Binh-Khiem, Nguyen, Takahata, Tomoyuki, Iwase, Eiji, Matsumoto, Kiyoshi, Shimoyama, Isao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247738/
https://www.ncbi.nlm.nih.gov/pubmed/22219693
http://dx.doi.org/10.3390/s100605888
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author Takamatsu, Seiichi
Takano, Hisanori
Binh-Khiem, Nguyen
Takahata, Tomoyuki
Iwase, Eiji
Matsumoto, Kiyoshi
Shimoyama, Isao
author_facet Takamatsu, Seiichi
Takano, Hisanori
Binh-Khiem, Nguyen
Takahata, Tomoyuki
Iwase, Eiji
Matsumoto, Kiyoshi
Shimoyama, Isao
author_sort Takamatsu, Seiichi
collection PubMed
description We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals.
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spelling pubmed-32477382012-01-04 Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors Takamatsu, Seiichi Takano, Hisanori Binh-Khiem, Nguyen Takahata, Tomoyuki Iwase, Eiji Matsumoto, Kiyoshi Shimoyama, Isao Sensors (Basel) Article We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals. Molecular Diversity Preservation International (MDPI) 2010-06-09 /pmc/articles/PMC3247738/ /pubmed/22219693 http://dx.doi.org/10.3390/s100605888 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Takamatsu, Seiichi
Takano, Hisanori
Binh-Khiem, Nguyen
Takahata, Tomoyuki
Iwase, Eiji
Matsumoto, Kiyoshi
Shimoyama, Isao
Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
title Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
title_full Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
title_fullStr Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
title_full_unstemmed Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
title_short Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
title_sort liquid-phase packaging of a glucose oxidase solution with parylene direct encapsulation and an ultraviolet curing adhesive cover for glucose sensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247738/
https://www.ncbi.nlm.nih.gov/pubmed/22219693
http://dx.doi.org/10.3390/s100605888
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