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Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247738/ https://www.ncbi.nlm.nih.gov/pubmed/22219693 http://dx.doi.org/10.3390/s100605888 |
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author | Takamatsu, Seiichi Takano, Hisanori Binh-Khiem, Nguyen Takahata, Tomoyuki Iwase, Eiji Matsumoto, Kiyoshi Shimoyama, Isao |
author_facet | Takamatsu, Seiichi Takano, Hisanori Binh-Khiem, Nguyen Takahata, Tomoyuki Iwase, Eiji Matsumoto, Kiyoshi Shimoyama, Isao |
author_sort | Takamatsu, Seiichi |
collection | PubMed |
description | We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals. |
format | Online Article Text |
id | pubmed-3247738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32477382012-01-04 Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors Takamatsu, Seiichi Takano, Hisanori Binh-Khiem, Nguyen Takahata, Tomoyuki Iwase, Eiji Matsumoto, Kiyoshi Shimoyama, Isao Sensors (Basel) Article We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals. Molecular Diversity Preservation International (MDPI) 2010-06-09 /pmc/articles/PMC3247738/ /pubmed/22219693 http://dx.doi.org/10.3390/s100605888 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Takamatsu, Seiichi Takano, Hisanori Binh-Khiem, Nguyen Takahata, Tomoyuki Iwase, Eiji Matsumoto, Kiyoshi Shimoyama, Isao Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors |
title | Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors |
title_full | Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors |
title_fullStr | Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors |
title_full_unstemmed | Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors |
title_short | Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors |
title_sort | liquid-phase packaging of a glucose oxidase solution with parylene direct encapsulation and an ultraviolet curing adhesive cover for glucose sensors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247738/ https://www.ncbi.nlm.nih.gov/pubmed/22219693 http://dx.doi.org/10.3390/s100605888 |
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