Optical Sensors Based on Whispering Gallery Modes in Fluorescent Microbeads: Response to Specific Interactions

Whispering gallery modes (WGMs) in surface-fixated fluorescent polystyrene microbeads are studied in view of their capability of sensing the formation of biochemical adsorption layers on their outer surface with the well-established biotin-streptavidin specific binding as the model system. Three dif...

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Detalles Bibliográficos
Autores principales: Himmelhaus, Michael, Krishnamoorthy, Sivashankar, Francois, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247756/
https://www.ncbi.nlm.nih.gov/pubmed/22219711
http://dx.doi.org/10.3390/s100606257
Descripción
Sumario:Whispering gallery modes (WGMs) in surface-fixated fluorescent polystyrene microbeads are studied in view of their capability of sensing the formation of biochemical adsorption layers on their outer surface with the well-established biotin-streptavidin specific binding as the model system. Three different methods for analysis of the observed shifts in the WGM wavelength positions are applied and used to quantify the adsorbed mass densities, which are then compared with the results of a comparative surface plasmon resonance (SPR) study.

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