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miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma

Based on the microRNA (miRNA) expression signatures of hypopharyngeal and esophageal squamous cell carcinoma, we found that miR-1 was significantly down-regulated in cancer cells. In this study, we investigated the functional significance of miR-1 in head and neck squamous cell carcinoma (HNSCC) cel...

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Autores principales: Nohata, Nijiro, Sone, Yaeko, Hanazawa, Toyoyuki, Fuse, Miki, Kikkawa, Naoko, Yoshino, Hirofumi, Chiyomaru, Takeshi, Kawakami, Kazumori, Enokida, Hideki, Nakagawa, Masayuki, Shozu, Makio, Okamoto, Yoshitaka, Seki, Naohiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248152/
https://www.ncbi.nlm.nih.gov/pubmed/21378409
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author Nohata, Nijiro
Sone, Yaeko
Hanazawa, Toyoyuki
Fuse, Miki
Kikkawa, Naoko
Yoshino, Hirofumi
Chiyomaru, Takeshi
Kawakami, Kazumori
Enokida, Hideki
Nakagawa, Masayuki
Shozu, Makio
Okamoto, Yoshitaka
Seki, Naohiko
author_facet Nohata, Nijiro
Sone, Yaeko
Hanazawa, Toyoyuki
Fuse, Miki
Kikkawa, Naoko
Yoshino, Hirofumi
Chiyomaru, Takeshi
Kawakami, Kazumori
Enokida, Hideki
Nakagawa, Masayuki
Shozu, Makio
Okamoto, Yoshitaka
Seki, Naohiko
author_sort Nohata, Nijiro
collection PubMed
description Based on the microRNA (miRNA) expression signatures of hypopharyngeal and esophageal squamous cell carcinoma, we found that miR-1 was significantly down-regulated in cancer cells. In this study, we investigated the functional significance of miR-1 in head and neck squamous cell carcinoma (HNSCC) cells and identified miR-1-regulated novel cancer pathways. Gain-of-function studies using miR-1 revealed significant decreases in HNSCC cell proliferation, invasion, and migration. In addition, the promotion of cell apoptosis and cell cycle arrest was demonstrated following miR-1 transfection of cancer cells. A search for the targets of miR-1 revealed that transgelin 2 (TAGLN2) was directly regulated by miR-1. Silencing of TAGLN2 significantly inhibited cell proliferation and invasion in HNSCC cells. Down-regulation of miR-1 and up-regulation of TAGLN2 were confirmed in HNSCC clinical specimens. Our data indicate that TAGLN2 may have an oncogenic function and may be regulated by miR-1, a tumor suppressive miRNA in HNSCC. The identification of novel miR-1-regulated cancer pathways could provide new insights into potential molecular mechanisms of HNSCC carcinogenesis.
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spelling pubmed-32481522012-01-18 miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma Nohata, Nijiro Sone, Yaeko Hanazawa, Toyoyuki Fuse, Miki Kikkawa, Naoko Yoshino, Hirofumi Chiyomaru, Takeshi Kawakami, Kazumori Enokida, Hideki Nakagawa, Masayuki Shozu, Makio Okamoto, Yoshitaka Seki, Naohiko Oncotarget Research Papers Based on the microRNA (miRNA) expression signatures of hypopharyngeal and esophageal squamous cell carcinoma, we found that miR-1 was significantly down-regulated in cancer cells. In this study, we investigated the functional significance of miR-1 in head and neck squamous cell carcinoma (HNSCC) cells and identified miR-1-regulated novel cancer pathways. Gain-of-function studies using miR-1 revealed significant decreases in HNSCC cell proliferation, invasion, and migration. In addition, the promotion of cell apoptosis and cell cycle arrest was demonstrated following miR-1 transfection of cancer cells. A search for the targets of miR-1 revealed that transgelin 2 (TAGLN2) was directly regulated by miR-1. Silencing of TAGLN2 significantly inhibited cell proliferation and invasion in HNSCC cells. Down-regulation of miR-1 and up-regulation of TAGLN2 were confirmed in HNSCC clinical specimens. Our data indicate that TAGLN2 may have an oncogenic function and may be regulated by miR-1, a tumor suppressive miRNA in HNSCC. The identification of novel miR-1-regulated cancer pathways could provide new insights into potential molecular mechanisms of HNSCC carcinogenesis. Impact Journals LLC 2011-01-25 /pmc/articles/PMC3248152/ /pubmed/21378409 Text en Copyright: © 2011 Nohata et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Papers
Nohata, Nijiro
Sone, Yaeko
Hanazawa, Toyoyuki
Fuse, Miki
Kikkawa, Naoko
Yoshino, Hirofumi
Chiyomaru, Takeshi
Kawakami, Kazumori
Enokida, Hideki
Nakagawa, Masayuki
Shozu, Makio
Okamoto, Yoshitaka
Seki, Naohiko
miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma
title miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma
title_full miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma
title_fullStr miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma
title_full_unstemmed miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma
title_short miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma
title_sort mir-1 as a tumor suppressive microrna targeting tagln2 in head and neck squamous cell carcinoma
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248152/
https://www.ncbi.nlm.nih.gov/pubmed/21378409
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