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Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells

Using the Hey3Met2 human ovarian cancer cell line, we previously found the RNASET2 gene to possess a remarkable in vivo tumor suppressor activity, although no in vitro features such as inhibition of cell proliferation, clonogenic potential, impaired growth in soft agar and increase in apoptotic rate...

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Autores principales: Acquati, Francesco, Monti, Laura, Lualdi, Marta, Fabbri, Marco, Sacco, Maria Grazia, Gribaldo, Laura, Taramelli, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248199/
https://www.ncbi.nlm.nih.gov/pubmed/21646684
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author Acquati, Francesco
Monti, Laura
Lualdi, Marta
Fabbri, Marco
Sacco, Maria Grazia
Gribaldo, Laura
Taramelli, Roberto
author_facet Acquati, Francesco
Monti, Laura
Lualdi, Marta
Fabbri, Marco
Sacco, Maria Grazia
Gribaldo, Laura
Taramelli, Roberto
author_sort Acquati, Francesco
collection PubMed
description Using the Hey3Met2 human ovarian cancer cell line, we previously found the RNASET2 gene to possess a remarkable in vivo tumor suppressor activity, although no in vitro features such as inhibition of cell proliferation, clonogenic potential, impaired growth in soft agar and increase in apoptotic rate could be detected. This is reminiscent of the behavior of genes belonging to the class of tumor antagonizing genes (TAG) which act mainly within the context of the microenvironment. Here we present transcriptional profiles analysis which indicates that investigations of the mechanisms of TAG biological functions require a comparison between the in vitro and in vivo expression patterns. Indeed several genes displaying a biological function potentially related to tumor suppression could not be validated by subsequent in vivo expression analysis. On the other hand the fact that we could find congruency for three genes both in vivo and in vitro adds a warning to a too much stringent categorization of this class of genes which relies on the sensitivity of the methodological approaches.
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spelling pubmed-32481992012-01-18 Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells Acquati, Francesco Monti, Laura Lualdi, Marta Fabbri, Marco Sacco, Maria Grazia Gribaldo, Laura Taramelli, Roberto Oncotarget Research Perspectives Using the Hey3Met2 human ovarian cancer cell line, we previously found the RNASET2 gene to possess a remarkable in vivo tumor suppressor activity, although no in vitro features such as inhibition of cell proliferation, clonogenic potential, impaired growth in soft agar and increase in apoptotic rate could be detected. This is reminiscent of the behavior of genes belonging to the class of tumor antagonizing genes (TAG) which act mainly within the context of the microenvironment. Here we present transcriptional profiles analysis which indicates that investigations of the mechanisms of TAG biological functions require a comparison between the in vitro and in vivo expression patterns. Indeed several genes displaying a biological function potentially related to tumor suppression could not be validated by subsequent in vivo expression analysis. On the other hand the fact that we could find congruency for three genes both in vivo and in vitro adds a warning to a too much stringent categorization of this class of genes which relies on the sensitivity of the methodological approaches. Impact Journals LLC 2011-06-04 /pmc/articles/PMC3248199/ /pubmed/21646684 Text en Copyright: © 2011 Acquati et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Perspectives
Acquati, Francesco
Monti, Laura
Lualdi, Marta
Fabbri, Marco
Sacco, Maria Grazia
Gribaldo, Laura
Taramelli, Roberto
Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells
title Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells
title_full Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells
title_fullStr Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells
title_full_unstemmed Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells
title_short Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells
title_sort molecular signature induced by rnaset2, a tumor antagonizing gene, in ovarian cancer cells
topic Research Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248199/
https://www.ncbi.nlm.nih.gov/pubmed/21646684
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