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Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation
BACKGROUND: In the past few years, cryopreservation of ovarian tissue has become an established procedure proposed in many centers around the world and transplantation has successfully resulted in full-term pregnancies and deliveries in human. This prospective study aims to evaluate the feasibility...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248844/ https://www.ncbi.nlm.nih.gov/pubmed/22112198 http://dx.doi.org/10.1186/1477-7827-9-150 |
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author | Fasano, Giovanna Moffa, Federica Dechène, Julie Englert, Yvon Demeestere, Isabelle |
author_facet | Fasano, Giovanna Moffa, Federica Dechène, Julie Englert, Yvon Demeestere, Isabelle |
author_sort | Fasano, Giovanna |
collection | PubMed |
description | BACKGROUND: In the past few years, cryopreservation of ovarian tissue has become an established procedure proposed in many centers around the world and transplantation has successfully resulted in full-term pregnancies and deliveries in human. This prospective study aims to evaluate the feasibility of vitrifying in vitro matured oocytes (IVM) isolated at the time of ovarian tissue cryopreservation to improve the efficiency of fertility preservation programs. METHODS: Oocyte-cumulus complexes were retrieved from freshly collected ovarian cortex by aspirating antral follicular fluid, and were matured in vitro for 24-48 h prior to vitrification. Oocytes were matured in an IVM commercial medium (Copper Surgical, USA) supplemented with 75 mIU/ml FSH and 75 mIU/ml LH and vitrified using a commercial vitrification kit (Irvine Scientific, California) in high security vitrification straws (CryoBioSystem, France). Oocyte collection and IVM rates were evaluated according to the age, the cycle period and the amount of tissue collected. RESULTS: Immature oocyte retrieval from ovarian tissue was carried out in 57 patients between 8 and 35 years of age, undergoing ovarian tissue cryopreservation. A total of 266 oocytes were isolated, 28 of them were degenerated, 200 were at germinal vesicle stage (GV), 35 were in metaphase I (MI) and 3 displayed a visible polar body (MII). The number of oocytes collected was positively correlated with the amount of tissue cryopreserved (p < 0.001) and negatively correlated with the age of the patients (p = 0.005). Oocytes were obtained regardless of menstrual cycle period or contraception. A total maturation rate of 31% was achieved, leading to the vitrification of at least one mature oocyte for half of the cohort. CONCLUSIONS: The study showed that a significant number of immature oocytes can be collected from excised ovarian tissue whatever the menstrual cycle phases and the age of the patients, even for prepubertal girls. |
format | Online Article Text |
id | pubmed-3248844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32488442011-12-31 Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation Fasano, Giovanna Moffa, Federica Dechène, Julie Englert, Yvon Demeestere, Isabelle Reprod Biol Endocrinol Research BACKGROUND: In the past few years, cryopreservation of ovarian tissue has become an established procedure proposed in many centers around the world and transplantation has successfully resulted in full-term pregnancies and deliveries in human. This prospective study aims to evaluate the feasibility of vitrifying in vitro matured oocytes (IVM) isolated at the time of ovarian tissue cryopreservation to improve the efficiency of fertility preservation programs. METHODS: Oocyte-cumulus complexes were retrieved from freshly collected ovarian cortex by aspirating antral follicular fluid, and were matured in vitro for 24-48 h prior to vitrification. Oocytes were matured in an IVM commercial medium (Copper Surgical, USA) supplemented with 75 mIU/ml FSH and 75 mIU/ml LH and vitrified using a commercial vitrification kit (Irvine Scientific, California) in high security vitrification straws (CryoBioSystem, France). Oocyte collection and IVM rates were evaluated according to the age, the cycle period and the amount of tissue collected. RESULTS: Immature oocyte retrieval from ovarian tissue was carried out in 57 patients between 8 and 35 years of age, undergoing ovarian tissue cryopreservation. A total of 266 oocytes were isolated, 28 of them were degenerated, 200 were at germinal vesicle stage (GV), 35 were in metaphase I (MI) and 3 displayed a visible polar body (MII). The number of oocytes collected was positively correlated with the amount of tissue cryopreserved (p < 0.001) and negatively correlated with the age of the patients (p = 0.005). Oocytes were obtained regardless of menstrual cycle period or contraception. A total maturation rate of 31% was achieved, leading to the vitrification of at least one mature oocyte for half of the cohort. CONCLUSIONS: The study showed that a significant number of immature oocytes can be collected from excised ovarian tissue whatever the menstrual cycle phases and the age of the patients, even for prepubertal girls. BioMed Central 2011-11-23 /pmc/articles/PMC3248844/ /pubmed/22112198 http://dx.doi.org/10.1186/1477-7827-9-150 Text en Copyright ©2011 Fasano et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Fasano, Giovanna Moffa, Federica Dechène, Julie Englert, Yvon Demeestere, Isabelle Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation |
title | Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation |
title_full | Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation |
title_fullStr | Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation |
title_full_unstemmed | Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation |
title_short | Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation |
title_sort | vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248844/ https://www.ncbi.nlm.nih.gov/pubmed/22112198 http://dx.doi.org/10.1186/1477-7827-9-150 |
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