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Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations

BACKGROUND: Differentiated thyroid carcinoma offers a good model to investigate the possible correlation between specific gene mutations and chromosome instability. Papillary thyroid neoplasms are characterized by different mutually exclusive genetic alterations, some of which are associated with an...

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Autores principales: Maric, Irena, Viaggi, Silvia, Caria, Paola, Frau, Daniela V, Degan, Paolo, Vanni, Roberta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248874/
https://www.ncbi.nlm.nih.gov/pubmed/22087789
http://dx.doi.org/10.1186/1755-8166-4-26
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author Maric, Irena
Viaggi, Silvia
Caria, Paola
Frau, Daniela V
Degan, Paolo
Vanni, Roberta
author_facet Maric, Irena
Viaggi, Silvia
Caria, Paola
Frau, Daniela V
Degan, Paolo
Vanni, Roberta
author_sort Maric, Irena
collection PubMed
description BACKGROUND: Differentiated thyroid carcinoma offers a good model to investigate the possible correlation between specific gene mutations and chromosome instability. Papillary thyroid neoplasms are characterized by different mutually exclusive genetic alterations, some of which are associated with aneuploidy and aggressive phenotype. RESULTS: We investigated the centrosome status and mitotic abnormalities in three thyroid carcinoma-derived cell lines, each maintaining the specific, biologically relevant gene alteration harbored by the parental tumors: RET/PTC1 rearrangement in TPC1; heterozygous and homozygous BRAF(V600E )mutation in K1 and in B-CPAP, respectively. B-CPAP cells showed a statistically significant (P < 0.01) higher frequency of abnormal mitotic figures compared to TPC1 and K1 cells. CONCLUSIONS: Our data indicate that RET/PTC1 oncogenic activity is not related to mitotic chromosome impairment and missegregation whereas, based on the consistent difference in types/frequencies of centrosome and spindle abnormalities observed between K1 and B-CPAP cells, the hetero/homozygous allelic status of BRAF(V600E )mutation seems to be not irrelevant in respect to chromosomal instability development.
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spelling pubmed-32488742011-12-31 Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations Maric, Irena Viaggi, Silvia Caria, Paola Frau, Daniela V Degan, Paolo Vanni, Roberta Mol Cytogenet Research BACKGROUND: Differentiated thyroid carcinoma offers a good model to investigate the possible correlation between specific gene mutations and chromosome instability. Papillary thyroid neoplasms are characterized by different mutually exclusive genetic alterations, some of which are associated with aneuploidy and aggressive phenotype. RESULTS: We investigated the centrosome status and mitotic abnormalities in three thyroid carcinoma-derived cell lines, each maintaining the specific, biologically relevant gene alteration harbored by the parental tumors: RET/PTC1 rearrangement in TPC1; heterozygous and homozygous BRAF(V600E )mutation in K1 and in B-CPAP, respectively. B-CPAP cells showed a statistically significant (P < 0.01) higher frequency of abnormal mitotic figures compared to TPC1 and K1 cells. CONCLUSIONS: Our data indicate that RET/PTC1 oncogenic activity is not related to mitotic chromosome impairment and missegregation whereas, based on the consistent difference in types/frequencies of centrosome and spindle abnormalities observed between K1 and B-CPAP cells, the hetero/homozygous allelic status of BRAF(V600E )mutation seems to be not irrelevant in respect to chromosomal instability development. BioMed Central 2011-11-16 /pmc/articles/PMC3248874/ /pubmed/22087789 http://dx.doi.org/10.1186/1755-8166-4-26 Text en Copyright ©2011 Maric et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Maric, Irena
Viaggi, Silvia
Caria, Paola
Frau, Daniela V
Degan, Paolo
Vanni, Roberta
Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
title Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
title_full Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
title_fullStr Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
title_full_unstemmed Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
title_short Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
title_sort centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248874/
https://www.ncbi.nlm.nih.gov/pubmed/22087789
http://dx.doi.org/10.1186/1755-8166-4-26
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