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Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model

BACKGROUND: Ulinastatin has anti-inflammatory properties and protects organs from ischemia/reperfusion-induced injury. The aim of this study was to investigate whether ulinastatin provides a protective effect on a regional myocardial ischemia/reperfusion injury in an in vivo rat heart model and to d...

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Autores principales: Shin, Il-Woo, Jang, In-Seok, Lee, Seung-Min, Park, Kyeong-Eon, Ok, Seong-Ho, Sohn, Ju-Tae, Lee, Heon-Keun, Chung, Young-Kyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Anesthesiologists 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249573/
https://www.ncbi.nlm.nih.gov/pubmed/22220228
http://dx.doi.org/10.4097/kjae.2011.61.6.499
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author Shin, Il-Woo
Jang, In-Seok
Lee, Seung-Min
Park, Kyeong-Eon
Ok, Seong-Ho
Sohn, Ju-Tae
Lee, Heon-Keun
Chung, Young-Kyun
author_facet Shin, Il-Woo
Jang, In-Seok
Lee, Seung-Min
Park, Kyeong-Eon
Ok, Seong-Ho
Sohn, Ju-Tae
Lee, Heon-Keun
Chung, Young-Kyun
author_sort Shin, Il-Woo
collection PubMed
description BACKGROUND: Ulinastatin has anti-inflammatory properties and protects organs from ischemia/reperfusion-induced injury. The aim of this study was to investigate whether ulinastatin provides a protective effect on a regional myocardial ischemia/reperfusion injury in an in vivo rat heart model and to determine whether the anti-inflammatory response is related to its myocardial protective effect. METHODS: Rats were randomized to two groups. One group is received ulinastatin (50,000 U/kg or 100,000 U/kg) diluted in normal saline and the other group is received normal saline, which was administered intraperitoneally 30 min before the ischemic insult. Reperfusion after 30 min of ischemia of the left coronary artery territory was applied. Hemodynamic measurements were recorded serially during 6 h after reperfusion. After the 6 h reperfusion, myocardial infarct size, cardiac enzymes, myeloperoxidase activity, and inflammatory cytokine levels were compared between the ulinastatin treated and untreated groups. RESULTS: Ulinastatin improved cardiac function and reduced infarct size after regional ischemia/reperfusion injury. Ulinastatin significantly attenuated tumor necrosis factor-α expression and reduced myeloperoxidase activity. CONCLUSIONS: Ulinastatin showed a myocardial protective effect after regional ischemia/reperfusion injury in an in vivo rat heart model. This protective effect of ulinastatin might be related in part to ulinastatin's ability to inhibit myeloperoxidase activity and decrease expression of tumor necrosis factor-α.
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spelling pubmed-32495732012-01-04 Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model Shin, Il-Woo Jang, In-Seok Lee, Seung-Min Park, Kyeong-Eon Ok, Seong-Ho Sohn, Ju-Tae Lee, Heon-Keun Chung, Young-Kyun Korean J Anesthesiol Experimental Research Article BACKGROUND: Ulinastatin has anti-inflammatory properties and protects organs from ischemia/reperfusion-induced injury. The aim of this study was to investigate whether ulinastatin provides a protective effect on a regional myocardial ischemia/reperfusion injury in an in vivo rat heart model and to determine whether the anti-inflammatory response is related to its myocardial protective effect. METHODS: Rats were randomized to two groups. One group is received ulinastatin (50,000 U/kg or 100,000 U/kg) diluted in normal saline and the other group is received normal saline, which was administered intraperitoneally 30 min before the ischemic insult. Reperfusion after 30 min of ischemia of the left coronary artery territory was applied. Hemodynamic measurements were recorded serially during 6 h after reperfusion. After the 6 h reperfusion, myocardial infarct size, cardiac enzymes, myeloperoxidase activity, and inflammatory cytokine levels were compared between the ulinastatin treated and untreated groups. RESULTS: Ulinastatin improved cardiac function and reduced infarct size after regional ischemia/reperfusion injury. Ulinastatin significantly attenuated tumor necrosis factor-α expression and reduced myeloperoxidase activity. CONCLUSIONS: Ulinastatin showed a myocardial protective effect after regional ischemia/reperfusion injury in an in vivo rat heart model. This protective effect of ulinastatin might be related in part to ulinastatin's ability to inhibit myeloperoxidase activity and decrease expression of tumor necrosis factor-α. The Korean Society of Anesthesiologists 2011-12 2011-12-20 /pmc/articles/PMC3249573/ /pubmed/22220228 http://dx.doi.org/10.4097/kjae.2011.61.6.499 Text en Copyright © the Korean Society of Anesthesiologists, 2011 http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Experimental Research Article
Shin, Il-Woo
Jang, In-Seok
Lee, Seung-Min
Park, Kyeong-Eon
Ok, Seong-Ho
Sohn, Ju-Tae
Lee, Heon-Keun
Chung, Young-Kyun
Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model
title Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model
title_full Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model
title_fullStr Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model
title_full_unstemmed Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model
title_short Myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model
title_sort myocardial protective effect by ulinastatin via an anti-inflammatory response after regional ischemia/reperfusion injury in an in vivo rat heart model
topic Experimental Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249573/
https://www.ncbi.nlm.nih.gov/pubmed/22220228
http://dx.doi.org/10.4097/kjae.2011.61.6.499
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