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Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment
It is well-recognized that vitamin D(3) has immune-modulatory properties and that the variation in ultraviolet (UV) exposure affects vitamin D(3) status. Here, we investigated if and to what extent seasonality of vitamin D(3) levels are associated with changes in T cell numbers and phenotypes. Every...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3250425/ https://www.ncbi.nlm.nih.gov/pubmed/22235276 http://dx.doi.org/10.1371/journal.pone.0029250 |
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author | Khoo, Ai-Leng Koenen, Hans J. P. M. Chai, Louis Y. A. Sweep, Fred C. G. J. Netea, Mihai G. van der Ven, André J. A. M. Joosten, Irma |
author_facet | Khoo, Ai-Leng Koenen, Hans J. P. M. Chai, Louis Y. A. Sweep, Fred C. G. J. Netea, Mihai G. van der Ven, André J. A. M. Joosten, Irma |
author_sort | Khoo, Ai-Leng |
collection | PubMed |
description | It is well-recognized that vitamin D(3) has immune-modulatory properties and that the variation in ultraviolet (UV) exposure affects vitamin D(3) status. Here, we investigated if and to what extent seasonality of vitamin D(3) levels are associated with changes in T cell numbers and phenotypes. Every three months during the course of the entire year, human PBMC and whole blood from 15 healthy subjects were sampled and analyzed using flow cytometry. We observed that elevated serum 25(OH)D(3) and 1,25(OH)(2)D(3) levels in summer were associated with a higher number of peripheral CD4(+) and CD8(+) T cells. In addition, an increase in naïve CD4(+)CD45RA(+) T cells with a reciprocal drop in memory CD4(+)CD45RO(+) T cells was observed. The increase in CD4(+)CD45RA(+) T cell count was a result of heightened proliferative capacity rather than recent thymic emigration of T cells. The percentage of Treg dropped in summer, but not the absolute Treg numbers. Notably, in the Treg population, the levels of forkhead box protein 3 (Foxp3) expression were increased in summer. Skin, gut and lymphoid tissue homing potential was increased during summer as well, exemplified by increased CCR4, CCR6, CLA, CCR9 and CCR7 levels. Also, in summer, CD4(+) and CD8(+) T cells revealed a reduced capacity to produce pro-inflammatory cytokines. In conclusion, seasonal variation in vitamin D(3) status in vivo throughout the year is associated with changes in the human peripheral T cell compartment and may as such explain some of the seasonal variation in immune status which has been observed previously. Given that the current observations are limited to healthy adult males, larger population-based studies would be useful to validate these findings. |
format | Online Article Text |
id | pubmed-3250425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32504252012-01-10 Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment Khoo, Ai-Leng Koenen, Hans J. P. M. Chai, Louis Y. A. Sweep, Fred C. G. J. Netea, Mihai G. van der Ven, André J. A. M. Joosten, Irma PLoS One Research Article It is well-recognized that vitamin D(3) has immune-modulatory properties and that the variation in ultraviolet (UV) exposure affects vitamin D(3) status. Here, we investigated if and to what extent seasonality of vitamin D(3) levels are associated with changes in T cell numbers and phenotypes. Every three months during the course of the entire year, human PBMC and whole blood from 15 healthy subjects were sampled and analyzed using flow cytometry. We observed that elevated serum 25(OH)D(3) and 1,25(OH)(2)D(3) levels in summer were associated with a higher number of peripheral CD4(+) and CD8(+) T cells. In addition, an increase in naïve CD4(+)CD45RA(+) T cells with a reciprocal drop in memory CD4(+)CD45RO(+) T cells was observed. The increase in CD4(+)CD45RA(+) T cell count was a result of heightened proliferative capacity rather than recent thymic emigration of T cells. The percentage of Treg dropped in summer, but not the absolute Treg numbers. Notably, in the Treg population, the levels of forkhead box protein 3 (Foxp3) expression were increased in summer. Skin, gut and lymphoid tissue homing potential was increased during summer as well, exemplified by increased CCR4, CCR6, CLA, CCR9 and CCR7 levels. Also, in summer, CD4(+) and CD8(+) T cells revealed a reduced capacity to produce pro-inflammatory cytokines. In conclusion, seasonal variation in vitamin D(3) status in vivo throughout the year is associated with changes in the human peripheral T cell compartment and may as such explain some of the seasonal variation in immune status which has been observed previously. Given that the current observations are limited to healthy adult males, larger population-based studies would be useful to validate these findings. Public Library of Science 2012-01-03 /pmc/articles/PMC3250425/ /pubmed/22235276 http://dx.doi.org/10.1371/journal.pone.0029250 Text en Khoo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Khoo, Ai-Leng Koenen, Hans J. P. M. Chai, Louis Y. A. Sweep, Fred C. G. J. Netea, Mihai G. van der Ven, André J. A. M. Joosten, Irma Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment |
title | Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment |
title_full | Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment |
title_fullStr | Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment |
title_full_unstemmed | Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment |
title_short | Seasonal Variation in Vitamin D(3) Levels Is Paralleled by Changes in the Peripheral Blood Human T Cell Compartment |
title_sort | seasonal variation in vitamin d(3) levels is paralleled by changes in the peripheral blood human t cell compartment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3250425/ https://www.ncbi.nlm.nih.gov/pubmed/22235276 http://dx.doi.org/10.1371/journal.pone.0029250 |
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