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GABA Expression and Regulation by Sensory Experience in the Developing Visual System
The developing retinotectal system of the Xenopus laevis tadpole is a model of choice for studying visual experience-dependent circuit maturation in the intact animal. The neurotransmitter gamma-aminobutyric acid (GABA) has been shown to play a critical role in the formation of sensory circuits in t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252287/ https://www.ncbi.nlm.nih.gov/pubmed/22242157 http://dx.doi.org/10.1371/journal.pone.0029086 |
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author | Miraucourt, Loïs S. da Silva, Jorge Santos Burgos, Kasandra Li, Jianli Abe, Hikari Ruthazer, Edward S. Cline, Hollis T. |
author_facet | Miraucourt, Loïs S. da Silva, Jorge Santos Burgos, Kasandra Li, Jianli Abe, Hikari Ruthazer, Edward S. Cline, Hollis T. |
author_sort | Miraucourt, Loïs S. |
collection | PubMed |
description | The developing retinotectal system of the Xenopus laevis tadpole is a model of choice for studying visual experience-dependent circuit maturation in the intact animal. The neurotransmitter gamma-aminobutyric acid (GABA) has been shown to play a critical role in the formation of sensory circuits in this preparation, however a comprehensive neuroanatomical study of GABAergic cell distribution in the developing tadpole has not been conducted. We report a detailed description of the spatial expression of GABA immunoreactivity in the Xenopus laevis tadpole brain at two key developmental stages: stage 40/42 around the onset of retinotectal innervation and stage 47 when the retinotectal circuit supports visually-guided behavior. During this period, GABAergic neurons within specific brain structures appeared to redistribute from clusters of neuronal somata to a sparser, more uniform distribution. Furthermore, we found that GABA levels were regulated by recent sensory experience. Both ELISA measurements of GABA concentration and quantitative analysis of GABA immunoreactivity in tissue sections from the optic tectum show that GABA increased in response to a 4 hr period of enhanced visual stimulation in stage 47 tadpoles. These observations reveal a remarkable degree of adaptability of GABAergic neurons in the developing brain, consistent with their key contributions to circuit development and function. |
format | Online Article Text |
id | pubmed-3252287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32522872012-01-12 GABA Expression and Regulation by Sensory Experience in the Developing Visual System Miraucourt, Loïs S. da Silva, Jorge Santos Burgos, Kasandra Li, Jianli Abe, Hikari Ruthazer, Edward S. Cline, Hollis T. PLoS One Research Article The developing retinotectal system of the Xenopus laevis tadpole is a model of choice for studying visual experience-dependent circuit maturation in the intact animal. The neurotransmitter gamma-aminobutyric acid (GABA) has been shown to play a critical role in the formation of sensory circuits in this preparation, however a comprehensive neuroanatomical study of GABAergic cell distribution in the developing tadpole has not been conducted. We report a detailed description of the spatial expression of GABA immunoreactivity in the Xenopus laevis tadpole brain at two key developmental stages: stage 40/42 around the onset of retinotectal innervation and stage 47 when the retinotectal circuit supports visually-guided behavior. During this period, GABAergic neurons within specific brain structures appeared to redistribute from clusters of neuronal somata to a sparser, more uniform distribution. Furthermore, we found that GABA levels were regulated by recent sensory experience. Both ELISA measurements of GABA concentration and quantitative analysis of GABA immunoreactivity in tissue sections from the optic tectum show that GABA increased in response to a 4 hr period of enhanced visual stimulation in stage 47 tadpoles. These observations reveal a remarkable degree of adaptability of GABAergic neurons in the developing brain, consistent with their key contributions to circuit development and function. Public Library of Science 2012-01-05 /pmc/articles/PMC3252287/ /pubmed/22242157 http://dx.doi.org/10.1371/journal.pone.0029086 Text en Miraucourt et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Miraucourt, Loïs S. da Silva, Jorge Santos Burgos, Kasandra Li, Jianli Abe, Hikari Ruthazer, Edward S. Cline, Hollis T. GABA Expression and Regulation by Sensory Experience in the Developing Visual System |
title | GABA Expression and Regulation by Sensory Experience in the Developing Visual System |
title_full | GABA Expression and Regulation by Sensory Experience in the Developing Visual System |
title_fullStr | GABA Expression and Regulation by Sensory Experience in the Developing Visual System |
title_full_unstemmed | GABA Expression and Regulation by Sensory Experience in the Developing Visual System |
title_short | GABA Expression and Regulation by Sensory Experience in the Developing Visual System |
title_sort | gaba expression and regulation by sensory experience in the developing visual system |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252287/ https://www.ncbi.nlm.nih.gov/pubmed/22242157 http://dx.doi.org/10.1371/journal.pone.0029086 |
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