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Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection

The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite tra...

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Autores principales: Dolezal, Pavel, Aili, Margareta, Tong, Janette, Jiang, Jhih-Hang, Marobbio, Carlo M., Lee, Sau fung, Schuelein, Ralf, Belluzzo, Simon, Binova, Eva, Mousnier, Aurelie, Frankel, Gad, Giannuzzi, Giulia, Palmieri, Ferdinando, Gabriel, Kipros, Naderer, Thomas, Hartland, Elizabeth L., Lithgow, Trevor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252375/
https://www.ncbi.nlm.nih.gov/pubmed/22241989
http://dx.doi.org/10.1371/journal.ppat.1002459
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author Dolezal, Pavel
Aili, Margareta
Tong, Janette
Jiang, Jhih-Hang
Marobbio, Carlo M.
Lee, Sau fung
Schuelein, Ralf
Belluzzo, Simon
Binova, Eva
Mousnier, Aurelie
Frankel, Gad
Giannuzzi, Giulia
Palmieri, Ferdinando
Gabriel, Kipros
Naderer, Thomas
Hartland, Elizabeth L.
Lithgow, Trevor
author_facet Dolezal, Pavel
Aili, Margareta
Tong, Janette
Jiang, Jhih-Hang
Marobbio, Carlo M.
Lee, Sau fung
Schuelein, Ralf
Belluzzo, Simon
Binova, Eva
Mousnier, Aurelie
Frankel, Gad
Giannuzzi, Giulia
Palmieri, Ferdinando
Gabriel, Kipros
Naderer, Thomas
Hartland, Elizabeth L.
Lithgow, Trevor
author_sort Dolezal, Pavel
collection PubMed
description The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. We discovered a novel MCF member, termed Legionella nucleotide carrier Protein (LncP), encoded in the genome of Legionella pneumophila, the causative agent of Legionnaire's disease. LncP was secreted via the bacterial Dot/Icm type IV secretion system into macrophages and assembled in the mitochondrial inner membrane. In a yeast cellular system, LncP induced a dominant-negative phenotype that was rescued by deleting an endogenous ATP carrier. Substrate transport studies on purified LncP reconstituted in liposomes revealed that it catalyzes unidirectional transport and exchange of ATP transport across membranes, thereby supporting a role for LncP as an ATP transporter. A hidden Markov model revealed further MCF proteins in the intracellular pathogens, Legionella longbeachae and Neorickettsia sennetsu, thereby challenging the notion that MCF proteins exist exclusively in eukaryotic organisms.
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spelling pubmed-32523752012-01-12 Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection Dolezal, Pavel Aili, Margareta Tong, Janette Jiang, Jhih-Hang Marobbio, Carlo M. Lee, Sau fung Schuelein, Ralf Belluzzo, Simon Binova, Eva Mousnier, Aurelie Frankel, Gad Giannuzzi, Giulia Palmieri, Ferdinando Gabriel, Kipros Naderer, Thomas Hartland, Elizabeth L. Lithgow, Trevor PLoS Pathog Research Article The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. We discovered a novel MCF member, termed Legionella nucleotide carrier Protein (LncP), encoded in the genome of Legionella pneumophila, the causative agent of Legionnaire's disease. LncP was secreted via the bacterial Dot/Icm type IV secretion system into macrophages and assembled in the mitochondrial inner membrane. In a yeast cellular system, LncP induced a dominant-negative phenotype that was rescued by deleting an endogenous ATP carrier. Substrate transport studies on purified LncP reconstituted in liposomes revealed that it catalyzes unidirectional transport and exchange of ATP transport across membranes, thereby supporting a role for LncP as an ATP transporter. A hidden Markov model revealed further MCF proteins in the intracellular pathogens, Legionella longbeachae and Neorickettsia sennetsu, thereby challenging the notion that MCF proteins exist exclusively in eukaryotic organisms. Public Library of Science 2012-01-05 /pmc/articles/PMC3252375/ /pubmed/22241989 http://dx.doi.org/10.1371/journal.ppat.1002459 Text en Dolezal et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dolezal, Pavel
Aili, Margareta
Tong, Janette
Jiang, Jhih-Hang
Marobbio, Carlo M.
Lee, Sau fung
Schuelein, Ralf
Belluzzo, Simon
Binova, Eva
Mousnier, Aurelie
Frankel, Gad
Giannuzzi, Giulia
Palmieri, Ferdinando
Gabriel, Kipros
Naderer, Thomas
Hartland, Elizabeth L.
Lithgow, Trevor
Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection
title Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection
title_full Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection
title_fullStr Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection
title_full_unstemmed Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection
title_short Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection
title_sort legionella pneumophila secretes a mitochondrial carrier protein during infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252375/
https://www.ncbi.nlm.nih.gov/pubmed/22241989
http://dx.doi.org/10.1371/journal.ppat.1002459
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