Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery

Supercoiled DNAs varying from 281 to 5302 bp were subjected to shear forces generated by aerosolization or sonication. DNA shearing strongly correlated with length. Typical sized plasmids (⩾3000 bp) degraded rapidly. DNAs 2000–3000 bp persisted ∼10 min. Even in the absence of condensing agents, supe...

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Detalles Bibliográficos
Autores principales: Catanese, D J, Fogg, J M, Schrock, D E, Gilbert, B E, Zechiedrich, L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252587/
https://www.ncbi.nlm.nih.gov/pubmed/21633394
http://dx.doi.org/10.1038/gt.2011.77
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author Catanese, D J
Fogg, J M
Schrock, D E
Gilbert, B E
Zechiedrich, L
author_facet Catanese, D J
Fogg, J M
Schrock, D E
Gilbert, B E
Zechiedrich, L
author_sort Catanese, D J
collection PubMed
description Supercoiled DNAs varying from 281 to 5302 bp were subjected to shear forces generated by aerosolization or sonication. DNA shearing strongly correlated with length. Typical sized plasmids (⩾3000 bp) degraded rapidly. DNAs 2000–3000 bp persisted ∼10 min. Even in the absence of condensing agents, supercoiled DNA <1200 bp survived nebulization, and increased forces of sonication were necessary to shear it. Circular vectors were considerably more resistant to shearing than linear vectors of the same length. DNA supercoiling afforded additional protection. These results show the potential of shear-resistant Minivector DNAs to overcome one of the major challenges associated with gene therapy delivery.
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spelling pubmed-32525872012-01-10 Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery Catanese, D J Fogg, J M Schrock, D E Gilbert, B E Zechiedrich, L Gene Ther Original Article Supercoiled DNAs varying from 281 to 5302 bp were subjected to shear forces generated by aerosolization or sonication. DNA shearing strongly correlated with length. Typical sized plasmids (⩾3000 bp) degraded rapidly. DNAs 2000–3000 bp persisted ∼10 min. Even in the absence of condensing agents, supercoiled DNA <1200 bp survived nebulization, and increased forces of sonication were necessary to shear it. Circular vectors were considerably more resistant to shearing than linear vectors of the same length. DNA supercoiling afforded additional protection. These results show the potential of shear-resistant Minivector DNAs to overcome one of the major challenges associated with gene therapy delivery. Nature Publishing Group 2012-01 2011-06-02 /pmc/articles/PMC3252587/ /pubmed/21633394 http://dx.doi.org/10.1038/gt.2011.77 Text en Copyright © 2012 Macmillan Publishers Limited http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Catanese, D J
Fogg, J M
Schrock, D E
Gilbert, B E
Zechiedrich, L
Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery
title Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery
title_full Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery
title_fullStr Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery
title_full_unstemmed Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery
title_short Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery
title_sort supercoiled minivector dna resists shear forces associated with gene therapy delivery
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252587/
https://www.ncbi.nlm.nih.gov/pubmed/21633394
http://dx.doi.org/10.1038/gt.2011.77
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