Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities.

Detalles Bibliográficos
Autores principales: Robin, Aélig, Köhler, Valentin, Jones, Alison, Ali, Afruja, Kelly, Paul P, O'Reilly, Elaine, Turner, Nicholas J, Flitsch, Sabine L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2011
Materias:
Full Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252848/
https://www.ncbi.nlm.nih.gov/pubmed/22238522
http://dx.doi.org/10.3762/bjoc.7.173
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author Robin, Aélig
Köhler, Valentin
Jones, Alison
Ali, Afruja
Kelly, Paul P
O'Reilly, Elaine
Turner, Nicholas J
Flitsch, Sabine L
author_facet Robin, Aélig
Köhler, Valentin
Jones, Alison
Ali, Afruja
Kelly, Paul P
O'Reilly, Elaine
Turner, Nicholas J
Flitsch, Sabine L
author_sort Robin, Aélig
collection PubMed
description A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities.
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spelling pubmed-32528482012-01-11 Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope Robin, Aélig Köhler, Valentin Jones, Alison Ali, Afruja Kelly, Paul P O'Reilly, Elaine Turner, Nicholas J Flitsch, Sabine L Beilstein J Org Chem Full Research Paper A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities. Beilstein-Institut 2011-11-02 /pmc/articles/PMC3252848/ /pubmed/22238522 http://dx.doi.org/10.3762/bjoc.7.173 Text en Copyright © 2011, Robin et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Full Research Paper
Robin, Aélig
Köhler, Valentin
Jones, Alison
Ali, Afruja
Kelly, Paul P
O'Reilly, Elaine
Turner, Nicholas J
Flitsch, Sabine L
Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope
title Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope
title_full Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope
title_fullStr Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope
title_full_unstemmed Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope
title_short Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope
title_sort chimeric self-sufficient p450cam-rhfred biocatalysts with broad substrate scope
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252848/
https://www.ncbi.nlm.nih.gov/pubmed/22238522
http://dx.doi.org/10.3762/bjoc.7.173
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