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The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina
The mouse retina is vascularized after birth when angiogenic blood vessels grow and sprout along a pre-formed latticework of astrocytes. How astrocyte-derived cues control patterns of blood vessel growth and sprouting, however, remains enigmatic. Here, we have used molecular genetic strategies in mi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Company of Biologists
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253036/ https://www.ncbi.nlm.nih.gov/pubmed/22069187 http://dx.doi.org/10.1242/dev.069153 |
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author | Hirota, Shinya Liu, Qian Lee, Hye Shin Hossain, Mohammad G. Lacy-Hulbert, Adam McCarty, Joseph H. |
author_facet | Hirota, Shinya Liu, Qian Lee, Hye Shin Hossain, Mohammad G. Lacy-Hulbert, Adam McCarty, Joseph H. |
author_sort | Hirota, Shinya |
collection | PubMed |
description | The mouse retina is vascularized after birth when angiogenic blood vessels grow and sprout along a pre-formed latticework of astrocytes. How astrocyte-derived cues control patterns of blood vessel growth and sprouting, however, remains enigmatic. Here, we have used molecular genetic strategies in mice to demonstrate that αvβ8 integrin expressed in astrocytes is essential for neovascularization of the developing retina. Selective ablation of αv or β8 integrin gene expression in astrocytes leads to impaired blood vessel sprouting and intraretinal hemorrhage, particularly during formation of the secondary vascular plexus. These pathologies correlate, in part, with diminished αvβ8 integrin-mediated activation of extracellular matrix-bound latent transforming growth factor βs (TGFβs) and defective TGFβ signaling in vascular endothelial cells, but not astrocytes. Collectively, our data demonstrate that αvβ8 integrin is a component of a paracrine signaling axis that links astrocytes to blood vessels and is essential for proper regulation of retinal angiogenesis. |
format | Online Article Text |
id | pubmed-3253036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-32530362012-01-10 The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina Hirota, Shinya Liu, Qian Lee, Hye Shin Hossain, Mohammad G. Lacy-Hulbert, Adam McCarty, Joseph H. Development Research Articles The mouse retina is vascularized after birth when angiogenic blood vessels grow and sprout along a pre-formed latticework of astrocytes. How astrocyte-derived cues control patterns of blood vessel growth and sprouting, however, remains enigmatic. Here, we have used molecular genetic strategies in mice to demonstrate that αvβ8 integrin expressed in astrocytes is essential for neovascularization of the developing retina. Selective ablation of αv or β8 integrin gene expression in astrocytes leads to impaired blood vessel sprouting and intraretinal hemorrhage, particularly during formation of the secondary vascular plexus. These pathologies correlate, in part, with diminished αvβ8 integrin-mediated activation of extracellular matrix-bound latent transforming growth factor βs (TGFβs) and defective TGFβ signaling in vascular endothelial cells, but not astrocytes. Collectively, our data demonstrate that αvβ8 integrin is a component of a paracrine signaling axis that links astrocytes to blood vessels and is essential for proper regulation of retinal angiogenesis. Company of Biologists 2011-12-01 /pmc/articles/PMC3253036/ /pubmed/22069187 http://dx.doi.org/10.1242/dev.069153 Text en © 2011. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms. |
spellingShingle | Research Articles Hirota, Shinya Liu, Qian Lee, Hye Shin Hossain, Mohammad G. Lacy-Hulbert, Adam McCarty, Joseph H. The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina |
title | The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina |
title_full | The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina |
title_fullStr | The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina |
title_full_unstemmed | The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina |
title_short | The astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina |
title_sort | astrocyte-expressed integrin αvβ8 governs blood vessel sprouting in the developing retina |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253036/ https://www.ncbi.nlm.nih.gov/pubmed/22069187 http://dx.doi.org/10.1242/dev.069153 |
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