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Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition

MicroRNA (miRNA) sponges are transcripts with repeated miRNA antisense sequences that can sequester miRNAs from endogenous targets. MiRNA sponges are valuable tools for miRNA loss-of-function studies both in vitro and in vivo. We developed a fast and flexible method to generate miRNA sponges and tes...

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Detalles Bibliográficos
Autores principales: Kluiver, Joost, Gibcus, Johan H., Hettinga, Chris, Adema, Annelies, Richter, Mareike K. S., Halsema, Nancy, Slezak-Prochazka, Izabella, Ding, Ye, Kroesen, Bart-Jan, van den Berg, Anke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253070/
https://www.ncbi.nlm.nih.gov/pubmed/22238599
http://dx.doi.org/10.1371/journal.pone.0029275