Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas

Eleven samples of DNA from choriocarcinomas were studied by high resolution CGH-array 244 K. They were studied after histopathological confirmation of the diagnosis, of the androgenic etiology and after a microsatellite marker analysis confirming the absence of contamination of tumor DNA from matern...

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Autores principales: Poaty, Henriette, Coullin, Philippe, Peko, Jean Félix, Dessen, Philippe, Diatta, Ange Lucien, Valent, Alexander, Leguern, Eric, Prévot, Sophie, Gombé-Mbalawa, Charles, Candelier, Jean-Jacques, Picard, Jean-Yves, Bernheim, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253784/
https://www.ncbi.nlm.nih.gov/pubmed/22253721
http://dx.doi.org/10.1371/journal.pone.0029426
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author Poaty, Henriette
Coullin, Philippe
Peko, Jean Félix
Dessen, Philippe
Diatta, Ange Lucien
Valent, Alexander
Leguern, Eric
Prévot, Sophie
Gombé-Mbalawa, Charles
Candelier, Jean-Jacques
Picard, Jean-Yves
Bernheim, Alain
author_facet Poaty, Henriette
Coullin, Philippe
Peko, Jean Félix
Dessen, Philippe
Diatta, Ange Lucien
Valent, Alexander
Leguern, Eric
Prévot, Sophie
Gombé-Mbalawa, Charles
Candelier, Jean-Jacques
Picard, Jean-Yves
Bernheim, Alain
author_sort Poaty, Henriette
collection PubMed
description Eleven samples of DNA from choriocarcinomas were studied by high resolution CGH-array 244 K. They were studied after histopathological confirmation of the diagnosis, of the androgenic etiology and after a microsatellite marker analysis confirming the absence of contamination of tumor DNA from maternal DNA. Three cell lines, BeWo, JAR, JEG were also studied by this high resolution pangenomic technique. According to aCGH analysis, the de novo choriocarcinomas exhibited simple chromosomal rearrangements or normal profiles. The cell lines showed various and complex chromosomal aberrations. 23 Minimal Critical Regions were defined that allowed us to list the genes that were potentially implicated. Among them, unusually high numbers of microRNA clusters and imprinted genes were observed.
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spelling pubmed-32537842012-01-17 Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas Poaty, Henriette Coullin, Philippe Peko, Jean Félix Dessen, Philippe Diatta, Ange Lucien Valent, Alexander Leguern, Eric Prévot, Sophie Gombé-Mbalawa, Charles Candelier, Jean-Jacques Picard, Jean-Yves Bernheim, Alain PLoS One Research Article Eleven samples of DNA from choriocarcinomas were studied by high resolution CGH-array 244 K. They were studied after histopathological confirmation of the diagnosis, of the androgenic etiology and after a microsatellite marker analysis confirming the absence of contamination of tumor DNA from maternal DNA. Three cell lines, BeWo, JAR, JEG were also studied by this high resolution pangenomic technique. According to aCGH analysis, the de novo choriocarcinomas exhibited simple chromosomal rearrangements or normal profiles. The cell lines showed various and complex chromosomal aberrations. 23 Minimal Critical Regions were defined that allowed us to list the genes that were potentially implicated. Among them, unusually high numbers of microRNA clusters and imprinted genes were observed. Public Library of Science 2012-01-09 /pmc/articles/PMC3253784/ /pubmed/22253721 http://dx.doi.org/10.1371/journal.pone.0029426 Text en Poaty et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Poaty, Henriette
Coullin, Philippe
Peko, Jean Félix
Dessen, Philippe
Diatta, Ange Lucien
Valent, Alexander
Leguern, Eric
Prévot, Sophie
Gombé-Mbalawa, Charles
Candelier, Jean-Jacques
Picard, Jean-Yves
Bernheim, Alain
Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas
title Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas
title_full Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas
title_fullStr Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas
title_full_unstemmed Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas
title_short Genome-Wide High-Resolution aCGH Analysis of Gestational Choriocarcinomas
title_sort genome-wide high-resolution acgh analysis of gestational choriocarcinomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253784/
https://www.ncbi.nlm.nih.gov/pubmed/22253721
http://dx.doi.org/10.1371/journal.pone.0029426
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