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The Role of γ-Tubulin in Centrosomal Microtubule Organization

As part of a multi-subunit ring complex, γ-tubulin has been shown to promote microtubule nucleation both in vitro and in vivo, and the structural properties of the complex suggest that it also seals the minus ends of the polymers with a conical cap. Cells depleted of γ-tubulin, however, still displa...

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Autores principales: O'Toole, Eileen, Greenan, Garrett, Lange, Karen I., Srayko, Martin, Müller-Reichert, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3254605/
https://www.ncbi.nlm.nih.gov/pubmed/22253783
http://dx.doi.org/10.1371/journal.pone.0029795
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author O'Toole, Eileen
Greenan, Garrett
Lange, Karen I.
Srayko, Martin
Müller-Reichert, Thomas
author_facet O'Toole, Eileen
Greenan, Garrett
Lange, Karen I.
Srayko, Martin
Müller-Reichert, Thomas
author_sort O'Toole, Eileen
collection PubMed
description As part of a multi-subunit ring complex, γ-tubulin has been shown to promote microtubule nucleation both in vitro and in vivo, and the structural properties of the complex suggest that it also seals the minus ends of the polymers with a conical cap. Cells depleted of γ-tubulin, however, still display many microtubules that participate in mitotic spindle assembly, suggesting that γ-tubulin is not absolutely required for microtubule nucleation in vivo, and raising questions about the function of the minus end cap. Here, we assessed the role of γ-tubulin in centrosomal microtubule organisation using three-dimensional reconstructions of γ-tubulin-depleted C. elegans embryos. We found that microtubule minus-end capping and the PCM component SPD-5 are both essential for the proper placement of microtubules in the centrosome. Our results further suggest that γ-tubulin and SPD-5 limit microtubule polymerization within the centrosome core, and we propose a model for how abnormal microtubule organization at the centrosome could indirectly affect centriole structure and daughter centriole replication.
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spelling pubmed-32546052012-01-17 The Role of γ-Tubulin in Centrosomal Microtubule Organization O'Toole, Eileen Greenan, Garrett Lange, Karen I. Srayko, Martin Müller-Reichert, Thomas PLoS One Research Article As part of a multi-subunit ring complex, γ-tubulin has been shown to promote microtubule nucleation both in vitro and in vivo, and the structural properties of the complex suggest that it also seals the minus ends of the polymers with a conical cap. Cells depleted of γ-tubulin, however, still display many microtubules that participate in mitotic spindle assembly, suggesting that γ-tubulin is not absolutely required for microtubule nucleation in vivo, and raising questions about the function of the minus end cap. Here, we assessed the role of γ-tubulin in centrosomal microtubule organisation using three-dimensional reconstructions of γ-tubulin-depleted C. elegans embryos. We found that microtubule minus-end capping and the PCM component SPD-5 are both essential for the proper placement of microtubules in the centrosome. Our results further suggest that γ-tubulin and SPD-5 limit microtubule polymerization within the centrosome core, and we propose a model for how abnormal microtubule organization at the centrosome could indirectly affect centriole structure and daughter centriole replication. Public Library of Science 2012-01-10 /pmc/articles/PMC3254605/ /pubmed/22253783 http://dx.doi.org/10.1371/journal.pone.0029795 Text en O'Toole et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
O'Toole, Eileen
Greenan, Garrett
Lange, Karen I.
Srayko, Martin
Müller-Reichert, Thomas
The Role of γ-Tubulin in Centrosomal Microtubule Organization
title The Role of γ-Tubulin in Centrosomal Microtubule Organization
title_full The Role of γ-Tubulin in Centrosomal Microtubule Organization
title_fullStr The Role of γ-Tubulin in Centrosomal Microtubule Organization
title_full_unstemmed The Role of γ-Tubulin in Centrosomal Microtubule Organization
title_short The Role of γ-Tubulin in Centrosomal Microtubule Organization
title_sort role of γ-tubulin in centrosomal microtubule organization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3254605/
https://www.ncbi.nlm.nih.gov/pubmed/22253783
http://dx.doi.org/10.1371/journal.pone.0029795
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