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In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylatio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3254685/ https://www.ncbi.nlm.nih.gov/pubmed/22058406 http://dx.doi.org/10.1093/jxb/err313 |
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author | Greco, Maria Chiappetta, Adriana Bruno, Leonardo Bitonti, Maria Beatrice |
author_facet | Greco, Maria Chiappetta, Adriana Bruno, Leonardo Bitonti, Maria Beatrice |
author_sort | Greco, Maria |
collection | PubMed |
description | In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively growing organs, under short- (6 h) and long- (2 d or 4 d) term and low (10 μM) and high (50 μM) doses of Cd, through a Methylation-Sensitive Amplification Polymorphism technique and an immunocytological approach, respectively. The expression of one member of the CHROMOMETHYLASE (CMT) family, a DNA methyltransferase, was also assessed by qRT-PCR. Nuclear chromatin ultrastructure was investigated by transmission electron microscopy. Cd treatment induced a DNA hypermethylation, as well as an up-regulation of CMT, indicating that de novo methylation did indeed occur. Moreover, a high dose of Cd led to a progressive heterochromatinization of interphase nuclei and apoptotic figures were also observed after long-term treatment. The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase. Such changes are linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin. Overall, the data show an epigenetic basis to the mechanism underlying Cd toxicity in plants. |
format | Online Article Text |
id | pubmed-3254685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32546852012-01-11 In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning Greco, Maria Chiappetta, Adriana Bruno, Leonardo Bitonti, Maria Beatrice J Exp Bot Research Papers In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively growing organs, under short- (6 h) and long- (2 d or 4 d) term and low (10 μM) and high (50 μM) doses of Cd, through a Methylation-Sensitive Amplification Polymorphism technique and an immunocytological approach, respectively. The expression of one member of the CHROMOMETHYLASE (CMT) family, a DNA methyltransferase, was also assessed by qRT-PCR. Nuclear chromatin ultrastructure was investigated by transmission electron microscopy. Cd treatment induced a DNA hypermethylation, as well as an up-regulation of CMT, indicating that de novo methylation did indeed occur. Moreover, a high dose of Cd led to a progressive heterochromatinization of interphase nuclei and apoptotic figures were also observed after long-term treatment. The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase. Such changes are linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin. Overall, the data show an epigenetic basis to the mechanism underlying Cd toxicity in plants. Oxford University Press 2012-01 2011-11-04 /pmc/articles/PMC3254685/ /pubmed/22058406 http://dx.doi.org/10.1093/jxb/err313 Text en © 2011 The Author(s). http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) |
spellingShingle | Research Papers Greco, Maria Chiappetta, Adriana Bruno, Leonardo Bitonti, Maria Beatrice In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning |
title | In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning |
title_full | In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning |
title_fullStr | In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning |
title_full_unstemmed | In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning |
title_short | In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning |
title_sort | in posidonia oceanica cadmium induces changes in dna methylation and chromatin patterning |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3254685/ https://www.ncbi.nlm.nih.gov/pubmed/22058406 http://dx.doi.org/10.1093/jxb/err313 |
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