Measurement of the traction force of biological cells by digital holography

The traction force produced by biological cells has been visualized as distortions in flexible substrata. We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts. Surface deformation and the cellular tractio...

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Detalles Bibliográficos
Autores principales: Yu, Xiao, Cross, Michael, Liu, Changgeng, Clark, David C., Haynie, Donald T., Kim, Myung K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Cell Studies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255332/
https://www.ncbi.nlm.nih.gov/pubmed/22254175
http://dx.doi.org/10.1364/BOE.3.000153
Descripción
Sumario:The traction force produced by biological cells has been visualized as distortions in flexible substrata. We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts. Surface deformation and the cellular traction force have been measured from phase profiles in a direct and straightforward manner. DH-QPM is shown to provide highly efficient and versatile means for quantitatively analyzing cellular motility.

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