Measurement of the traction force of biological cells by digital holography

The traction force produced by biological cells has been visualized as distortions in flexible substrata. We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts. Surface deformation and the cellular tractio...

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Detalles Bibliográficos
Autores principales: Yu, Xiao, Cross, Michael, Liu, Changgeng, Clark, David C., Haynie, Donald T., Kim, Myung K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Cell Studies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255332/
https://www.ncbi.nlm.nih.gov/pubmed/22254175
http://dx.doi.org/10.1364/BOE.3.000153
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author Yu, Xiao
Cross, Michael
Liu, Changgeng
Clark, David C.
Haynie, Donald T.
Kim, Myung K.
author_facet Yu, Xiao
Cross, Michael
Liu, Changgeng
Clark, David C.
Haynie, Donald T.
Kim, Myung K.
author_sort Yu, Xiao
collection PubMed
description The traction force produced by biological cells has been visualized as distortions in flexible substrata. We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts. Surface deformation and the cellular traction force have been measured from phase profiles in a direct and straightforward manner. DH-QPM is shown to provide highly efficient and versatile means for quantitatively analyzing cellular motility.
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spelling pubmed-32553322012-01-17 Measurement of the traction force of biological cells by digital holography Yu, Xiao Cross, Michael Liu, Changgeng Clark, David C. Haynie, Donald T. Kim, Myung K. Biomed Opt Express Cell Studies The traction force produced by biological cells has been visualized as distortions in flexible substrata. We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts. Surface deformation and the cellular traction force have been measured from phase profiles in a direct and straightforward manner. DH-QPM is shown to provide highly efficient and versatile means for quantitatively analyzing cellular motility. Optical Society of America 2011-12-16 /pmc/articles/PMC3255332/ /pubmed/22254175 http://dx.doi.org/10.1364/BOE.3.000153 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Cell Studies
Yu, Xiao
Cross, Michael
Liu, Changgeng
Clark, David C.
Haynie, Donald T.
Kim, Myung K.
Measurement of the traction force of biological cells by digital holography
title Measurement of the traction force of biological cells by digital holography
title_full Measurement of the traction force of biological cells by digital holography
title_fullStr Measurement of the traction force of biological cells by digital holography
title_full_unstemmed Measurement of the traction force of biological cells by digital holography
title_short Measurement of the traction force of biological cells by digital holography
title_sort measurement of the traction force of biological cells by digital holography
topic Cell Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255332/
https://www.ncbi.nlm.nih.gov/pubmed/22254175
http://dx.doi.org/10.1364/BOE.3.000153
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