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Extending vaterite microviscometry to ex vivo blood vessels by serial calibration
The endothelial glycocalyx layer is a ~2 µm thick glycosaminoglycan rich pericellular matrix expressed on the luminal surface of vascular endothelial cells, which has implications in vessel mechanics and mechanotransduction. Despite its role in vascular physiology, no direct measurement has of yet b...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255340/ https://www.ncbi.nlm.nih.gov/pubmed/22254166 http://dx.doi.org/10.1364/BOE.3.000037 |
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author | Shreim, Samir G. Steward, Earl Botvinick, Elliot L. |
author_facet | Shreim, Samir G. Steward, Earl Botvinick, Elliot L. |
author_sort | Shreim, Samir G. |
collection | PubMed |
description | The endothelial glycocalyx layer is a ~2 µm thick glycosaminoglycan rich pericellular matrix expressed on the luminal surface of vascular endothelial cells, which has implications in vessel mechanics and mechanotransduction. Despite its role in vascular physiology, no direct measurement has of yet been made of vessel glycocalyx material properties. Vaterite microviscometry is a laser tweezers based microrheological method, which has been previously utilized to measure the viscosity of linear and complex fluids under flow. This form of microrheology has until now relied on complete recollection of the forward scattered light. Here we present a novel method to extend vaterite microviscometry to relatively thick samples. We validate our method and its assumptions and measure the apparent viscosity as a function of distance from the vascular endothelium. We observe a differential response in conditions designed to preserve the EGL in comparison to those designed to collapse it. |
format | Online Article Text |
id | pubmed-3255340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-32553402012-01-17 Extending vaterite microviscometry to ex vivo blood vessels by serial calibration Shreim, Samir G. Steward, Earl Botvinick, Elliot L. Biomed Opt Express Optical Traps, Manipulation, and Tracking The endothelial glycocalyx layer is a ~2 µm thick glycosaminoglycan rich pericellular matrix expressed on the luminal surface of vascular endothelial cells, which has implications in vessel mechanics and mechanotransduction. Despite its role in vascular physiology, no direct measurement has of yet been made of vessel glycocalyx material properties. Vaterite microviscometry is a laser tweezers based microrheological method, which has been previously utilized to measure the viscosity of linear and complex fluids under flow. This form of microrheology has until now relied on complete recollection of the forward scattered light. Here we present a novel method to extend vaterite microviscometry to relatively thick samples. We validate our method and its assumptions and measure the apparent viscosity as a function of distance from the vascular endothelium. We observe a differential response in conditions designed to preserve the EGL in comparison to those designed to collapse it. Optical Society of America 2011-12-05 /pmc/articles/PMC3255340/ /pubmed/22254166 http://dx.doi.org/10.1364/BOE.3.000037 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Optical Traps, Manipulation, and Tracking Shreim, Samir G. Steward, Earl Botvinick, Elliot L. Extending vaterite microviscometry to ex vivo blood vessels by serial calibration |
title | Extending vaterite microviscometry to ex vivo blood vessels by serial calibration |
title_full | Extending vaterite microviscometry to ex vivo blood vessels by serial calibration |
title_fullStr | Extending vaterite microviscometry to ex vivo blood vessels by serial calibration |
title_full_unstemmed | Extending vaterite microviscometry to ex vivo blood vessels by serial calibration |
title_short | Extending vaterite microviscometry to ex vivo blood vessels by serial calibration |
title_sort | extending vaterite microviscometry to ex vivo blood vessels by serial calibration |
topic | Optical Traps, Manipulation, and Tracking |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255340/ https://www.ncbi.nlm.nih.gov/pubmed/22254166 http://dx.doi.org/10.1364/BOE.3.000037 |
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