TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW

Bioanalysis frequently involves the measurement of very low analyte concentrations in complex and potentially variable matrices. An initial attempt has been made to apply a risk management tool to the bioanalytical method development like selection of spiked plasma volume, selection of internal stan...

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Autores principales: Sharma, Ganesh N., Singhal, Man Mohan, Sharma, K.K., Sanadya, Jyotsana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2010
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255387/
https://www.ncbi.nlm.nih.gov/pubmed/22247825
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author Sharma, Ganesh N.
Singhal, Man Mohan
Sharma, K.K.
Sanadya, Jyotsana
author_facet Sharma, Ganesh N.
Singhal, Man Mohan
Sharma, K.K.
Sanadya, Jyotsana
author_sort Sharma, Ganesh N.
collection PubMed
description Bioanalysis frequently involves the measurement of very low analyte concentrations in complex and potentially variable matrices. An initial attempt has been made to apply a risk management tool to the bioanalytical method development like selection of spiked plasma volume, selection of internal standard to minimize processing error, selection of medium and extraction procedure, setting of mobile phase and pH, determination of chromatographic conditions etc. and to minimize instrumental error like; ion suppression and matrix effect.
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spelling pubmed-32553872012-01-13 TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW Sharma, Ganesh N. Singhal, Man Mohan Sharma, K.K. Sanadya, Jyotsana J Adv Pharm Technol Res Review Article Bioanalysis frequently involves the measurement of very low analyte concentrations in complex and potentially variable matrices. An initial attempt has been made to apply a risk management tool to the bioanalytical method development like selection of spiked plasma volume, selection of internal standard to minimize processing error, selection of medium and extraction procedure, setting of mobile phase and pH, determination of chromatographic conditions etc. and to minimize instrumental error like; ion suppression and matrix effect. Medknow Publications & Media Pvt Ltd 2010 /pmc/articles/PMC3255387/ /pubmed/22247825 Text en Copyright: © Journal of Advanced Pharmaceutical Technology & Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Sharma, Ganesh N.
Singhal, Man Mohan
Sharma, K.K.
Sanadya, Jyotsana
TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW
title TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW
title_full TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW
title_fullStr TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW
title_full_unstemmed TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW
title_short TROUBLE SHOOTING DURING BIOANALYTICAL ESTIMATION OF DRUG AND METABOLITES USING LC-MS/MS: A REVIEW
title_sort trouble shooting during bioanalytical estimation of drug and metabolites using lc-ms/ms: a review
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255387/
https://www.ncbi.nlm.nih.gov/pubmed/22247825
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